Detailed information of TA system TA058786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3688893..3689567
• Replicon: chromosome
• Accession: NZ_CP013475
• Organism: Mycobacterium tuberculosis strain 1458

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: BTB1458_RS17500
• Protein ID: WP_003417282.1
• Coordinates: 3688893..3689321 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: BTB1458_RS17505
• Protein ID: WP_003417286.1
• Coordinates: 3689325..3689567 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTB1458_RS17475	3684715..3685116	-	402	WP_003417264.1	cytidine deaminase	-
BTB1458_RS17480	3685257..3685691	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
BTB1458_RS17485	3685688..3686122	+	435	WP_003902441.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
BTB1458_RS17490	3686251..3688023	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
BTB1458_RS17495	3688023..3688814	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
BTB1458_RS17500	3688893..3689321	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
BTB1458_RS17505	3689325..3689567	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
BTB1458_RS17510	3689689..3690303	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
BTB1458_RS17515	3690300..3690965	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
BTB1458_RS17520	3690966..3691499	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
BTB1458_RS17525	3691496..3691870	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
BTB1458_RS17530	3691967..3693031	-	1065	WP_079157054.1	GTP 3',8-cyclase MoaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T58786 WP_003417282.1 NZ_CP013475:c3689321-3688893 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T58786 NZ_CP013475:c3689321-3688893 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT58786 WP_003417286.1 NZ_CP013475:c3689567-3689325 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT58786 NZ_CP013475:c3689567-3689325 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59