Detailed information of TA system TA058782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3112273..3112877
• Replicon: chromosome
• Accession: NZ_CP013475
• Organism: Mycobacterium tuberculosis strain 1458

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: BTB1458_RS14845
• Protein ID: WP_003414492.1
• Coordinates: 3112273..3112665 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: BTB1458_RS14850
• Protein ID: WP_003414495.1
• Coordinates: 3112662..3112877 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTB1458_RS14815	3107423..3108211	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
BTB1458_RS14820	3108545..3109090	-	546	WP_014584866.1	DUF1802 family protein	-
BTB1458_RS14825	3109362..3110246	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
BTB1458_RS14830	3110249..3111136	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
BTB1458_RS14835	3111441..3111986	-	546	WP_003910939.1	DUF1802 family protein	-
BTB1458_RS14840	3111983..3112252	-	270	WP_003414489.1	DUF2277 family protein	-
BTB1458_RS14845	3112273..3112665	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
BTB1458_RS14850	3112662..3112877	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BTB1458_RS14855	3112924..3113673	+	750	WP_003902358.1	enoyl-CoA hydratase	-
BTB1458_RS14860	3113752..3114834	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
BTB1458_RS14865	3114827..3116137	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
BTB1458_RS14870	3116140..3116967	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
BTB1458_RS14875	3116964..3117875	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T58782 WP_003414492.1 NZ_CP013475:c3112665-3112273 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T58782 NZ_CP013475:c3112665-3112273 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT58782 WP_003414495.1 NZ_CP013475:c3112877-3112662 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT58782 NZ_CP013475:c3112877-3112662 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8