Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2852795..2853432 Replicon chromosome
Accession NZ_CP013475
Organism Mycobacterium tuberculosis strain 1458

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag BTB1458_RS13430 Protein ID WP_003413180.1
Coordinates 2852795..2853190 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag BTB1458_RS13435 Protein ID WP_003413183.1
Coordinates 2853187..2853432 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTB1458_RS13390 2848246..2848560 + 315 WP_009937839.1 hypothetical protein -
BTB1458_RS13395 2848856..2850067 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BTB1458_RS13400 2850194..2850855 + 662 Protein_2616 hypothetical protein -
BTB1458_RS22050 2850852..2851129 + 278 Protein_2617 type II toxin-antitoxin system VapB family antitoxin -
BTB1458_RS13410 2851222..2851635 + 414 WP_003413164.1 PIN domain nuclease -
BTB1458_RS13415 2851674..2851931 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
BTB1458_RS13420 2851928..2852305 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
BTB1458_RS13425 2852321..2852695 - 375 WP_003413177.1 hypothetical protein -
BTB1458_RS13430 2852795..2853190 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
BTB1458_RS13435 2853187..2853432 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
BTB1458_RS13440 2853843..2854262 - 420 WP_003413190.1 A24 family peptidase -
BTB1458_RS13445 2854274..2855083 - 810 WP_003413193.1 shikimate dehydrogenase -
BTB1458_RS13450 2855080..2856333 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BTB1458_RS13455 2856326..2856838 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T58775 WP_003413180.1 NZ_CP013475:c2853190-2852795 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T58775 NZ_CP013475:c2853190-2852795 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT58775 WP_003413183.1 NZ_CP013475:c2853432-2853187 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT58775 NZ_CP013475:c2853432-2853187 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References