Detailed information of TA system TA058760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2175870..2176573
• Replicon: chromosome
• Accession: NZ_CP013475
• Organism: Mycobacterium tuberculosis strain 1458

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BTB1458_RS10120
• Protein ID: WP_003409778.1
• Coordinates: 2175870..2176199 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BTB1458_RS21250
• Protein ID: WP_003409780.1
• Coordinates: 2176196..2176573 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTB1458_RS10100	2172253..2173323	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
BTB1458_RS10105	2173320..2173835	+	516	WP_003409718.1	flavin reductase family protein	-
BTB1458_RS10110	2173832..2174893	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BTB1458_RS10115	2174890..2175660	+	771	WP_003409775.1	SDR family oxidoreductase	-
BTB1458_RS10120	2175870..2176199	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BTB1458_RS21250	2176196..2176573	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BTB1458_RS10130	2176570..2177160	-	591	WP_003409784.1	SEC-C domain-containing protein	-
BTB1458_RS10135	2177215..2178579	+	1365	WP_003903691.1	HNH endonuclease	-
BTB1458_RS10140	2178734..2179186	-	453	WP_003899095.1	lipoprotein	-
BTB1458_RS10145	2179250..2179651	+	402	WP_003409869.1	hypothetical protein	-
BTB1458_RS10150	2179644..2179826	-	183	WP_003409870.1	hypothetical protein	-
BTB1458_RS10155	2179940..2180290	-	351	WP_003409871.1	hypothetical protein	-
BTB1458_RS10160	2180301..2181203	-	903	WP_003899097.1	hypothetical protein	-
BTB1458_RS10165	2181224..2181415	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T58760 WP_003409778.1 NZ_CP013475:c2176199-2175870 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T58760 NZ_CP013475:c2176199-2175870 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT58760 WP_003409780.1 NZ_CP013475:c2176573-2176196 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT58760 NZ_CP013475:c2176573-2176196 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8