Detailed information of TA system TA058729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 2549218..2549918
• Replicon: chromosome
• Accession: NZ_CP013467
• Organism: Burkholderia multivorans strain MSMB1640WGS

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A2S9M997
• Locus tag: WM33_RS22260
• Protein ID: WP_035951940.1
• Coordinates: 2549218..2549595 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: WM33_RS22265
• Protein ID: WP_006413467.1
• Coordinates: 2549595..2549918 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM33_RS22235	2544759..2545208	+	450	WP_006413500.1	VOC family protein	-
WM33_RS22240	2545312..2546862	-	1551	WP_006398698.1	sodium:solute symporter family protein	-
WM33_RS22245	2546859..2547092	-	234	WP_006398697.1	DUF3311 domain-containing protein	-
WM33_RS22250	2547289..2548206	-	918	WP_048805889.1	spermidine synthase	-
WM33_RS22255	2548434..2548688	+	255	WP_048805888.1	hypothetical protein	-
WM33_RS32910	2548790..2548927	+	138	WP_155772743.1	hypothetical protein	-
WM33_RS22260	2549218..2549595	+	378	WP_035951940.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WM33_RS22265	2549595..2549918	+	324	WP_006413467.1	helix-turn-helix domain-containing protein	Antitoxin
WM33_RS22270	2549941..2550432	-	492	WP_048805887.1	DNA-deoxyinosine glycosylase	-
WM33_RS22275	2550429..2551043	-	615	WP_012212956.1	chorismate lyase	-
WM33_RS22280	2551052..2552950	-	1899	WP_048805886.1	molecular chaperone HtpG	-
WM33_RS22285	2553110..2554570	-	1461	WP_048805885.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (23-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13604.61 Da
• Isoelectric Point: 9.5844

>T58729 WP_035951940.1 NZ_CP013467:2549218-2549595 [Burkholderia multivorans]
MGINSSMVPYTKPLYWLGSARRDLKAMPEAVQDTFGYALYLAQTGGKHDQAKPLRGFGSAGVLEVVESEDSGTYRAVYTV
KLGGAVYVLHCFQKKSCRGIATPKPDIELVRARLRAAEAHANGEK

Nucleotide

• Length: 378 bp

>T58729 NZ_CP013467:2549218-2549595 [Burkholderia multivorans]
ATGGGTATAAATTCGTCGATGGTTCCATACACTAAACCGCTTTACTGGCTCGGCTCGGCACGCAGGGACTTGAAAGCGAT
GCCCGAAGCGGTACAGGACACGTTTGGCTATGCGCTGTATCTGGCTCAGACGGGAGGAAAGCACGATCAGGCCAAGCCGT
TGAGGGGCTTCGGATCGGCCGGCGTGCTCGAGGTCGTGGAGTCGGAGGACAGCGGCACGTACCGGGCGGTCTATACGGTG
AAACTCGGTGGCGCCGTCTATGTGCTGCACTGTTTCCAGAAGAAATCGTGCCGCGGTATCGCTACACCGAAGCCGGACAT
CGAACTGGTTCGCGCGCGCCTGAGGGCCGCCGAAGCACACGCAAATGGAGAAAAGTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11828.75 Da
• Isoelectric Point: 8.0686

>AT58729 WP_006413467.1 NZ_CP013467:2549595-2549918 [Burkholderia multivorans]
MIEIERGSGNVYADLGMPDAEEMRVKAQLAAKIAEIIKTRKWTQQHAARLLGMTQPKLSNMLRGQFRGVSEAKMLDCLAK
LGRDVQIVVGPDRHSEAEGHIEVVFAA

Nucleotide

• Length: 324 bp

>AT58729 NZ_CP013467:2549595-2549918 [Burkholderia multivorans]
ATGATCGAAATCGAACGGGGCAGCGGCAACGTCTACGCGGATCTGGGCATGCCCGACGCCGAAGAAATGCGCGTCAAGGC
TCAACTCGCGGCCAAGATCGCGGAGATCATCAAGACCCGCAAATGGACGCAGCAGCACGCGGCGCGGTTGCTCGGCATGA
CGCAACCGAAGCTATCGAATATGCTGCGCGGGCAGTTTCGCGGCGTAAGCGAAGCGAAGATGCTCGACTGTCTCGCCAAG
CTCGGGCGCGATGTCCAGATCGTCGTAGGACCGGACCGTCATTCGGAAGCCGAAGGACACATCGAAGTCGTATTCGCTGC
CTGA

Structures

Toxin

• AlphaFold DB: A0A2S9M997