Detailed information of TA system TA058725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1726060..1726751
• Replicon: chromosome
• Accession: NZ_CP013466
• Organism: Burkholderia multivorans strain MSMB1640WGS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WM33_RS07430
• Protein ID: WP_048804531.1
• Coordinates: 1726060..1726515 (-)
• Length: 152 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A132F1C7
• Locus tag: WM33_RS07435
• Protein ID: WP_012216644.1
• Coordinates: 1726512..1726751 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM33_RS07405	1721178..1721972	+	795	WP_048804534.1	AraC family transcriptional regulator	-
WM33_RS07410	1722468..1723418	+	951	WP_080746542.1	DMT family transporter	-
WM33_RS07415	1723629..1724204	+	576	WP_048804532.1	hypothetical protein	-
WM33_RS07420	1724300..1724434	-	135	WP_006396654.1	entericidin A/B family lipoprotein	-
WM33_RS07425	1724667..1725962	+	1296	WP_060139956.1	aspartate carbamoyltransferase	-
WM33_RS07430	1726060..1726515	-	456	WP_048804531.1	type II toxin-antitoxin system VapC family toxin	Toxin
WM33_RS07435	1726512..1726751	-	240	WP_012216644.1	Arc family DNA-binding protein	Antitoxin
WM33_RS07445	1727055..1729301	+	2247	WP_048804529.1	TonB-dependent siderophore receptor	-
WM33_RS07450	1729305..1730060	+	756	WP_048804528.1	sel1 repeat family protein	-
WM33_RS07455	1730057..1730740	+	684	WP_035955277.1	Fe2+-dependent dioxygenase	-
WM33_RS07460	1731146..1731385	+	240	WP_006416205.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 152 a.a.
• Molecular weight: 16064.40 Da
• Isoelectric Point: 7.2060

>T58725 WP_048804531.1 NZ_CP013466:c1726515-1726060 [Burkholderia multivorans]
MILVDTNVIAEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLAQSIEHRVVPVFRGRILPFDD
AASKAYARIRAHARAGGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPGASQGAPVRQGIASK

Nucleotide

• Length: 456 bp

>T58725 NZ_CP013466:c1726515-1726060 [Burkholderia multivorans]
ATGATCCTTGTTGACACGAACGTCATTGCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
ACAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTGGCGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGAGCAAGGCCTATGCGCGCATTCGCGCGCACGCCCGTGCCGGCGGCAACGCGATCGCGCCCGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGGGGGCGTCGCAGGGCGCGCCGGTGCGCCAAGGTATCGCGTCGAAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8716.94 Da
• Isoelectric Point: 5.1133

>AT58725 WP_012216644.1 NZ_CP013466:c1726751-1726512 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSTEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTPTDPMSFE

Nucleotide

• Length: 240 bp

>AT58725 NZ_CP013466:c1726751-1726512 [Burkholderia multivorans]
ATGCCGGTGATTACCGTTCGAAATTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCGCTGCACGGGCGCAG
TACGGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGCGTGAAGCTCGGAACCTTGCTGGCGG
AAATCGGGCGGGAGGCAGGCGGTGTCGATCTCGACATTCAGCGTGACAAGACGCCAACCGATCCGATGAGCTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A132F1C7