Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 938618..939195 Replicon chromosome
Accession NZ_CP013466
Organism Burkholderia multivorans strain MSMB1640WGS

Toxin (Protein)


Gene name vapC Uniprot ID A0A2S9ML41
Locus tag WM33_RS04215 Protein ID WP_035954580.1
Coordinates 938618..938983 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2S9ML45
Locus tag WM33_RS04220 Protein ID WP_035956044.1
Coordinates 938977..939195 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM33_RS04190 933779..934678 + 900 WP_048803769.1 lipid A hydroxylase LpxO -
WM33_RS04195 934802..935536 + 735 WP_196755237.1 hypothetical protein -
WM33_RS04200 935597..937195 - 1599 WP_048803767.1 alkyl hydroperoxide reductase subunit F -
WM33_RS04205 937314..937877 - 564 WP_006399806.1 peroxiredoxin -
WM33_RS04210 938133..938567 + 435 WP_009688132.1 GNAT family N-acetyltransferase -
WM33_RS04215 938618..938983 - 366 WP_035954580.1 type II toxin-antitoxin system VapC family toxin Toxin
WM33_RS04220 938977..939195 - 219 WP_035956044.1 hypothetical protein Antitoxin
WM33_RS04225 939297..940193 - 897 WP_048803766.1 LysR family transcriptional regulator -
WM33_RS04230 940369..941535 + 1167 WP_048803765.1 branched-chain amino acid ABC transporter substrate-binding protein -
WM33_RS04235 941753..942289 + 537 WP_060139603.1 L-2-amino-thiazoline-4-carboxylic acid hydrolase -
WM33_RS04240 942282..943553 + 1272 WP_048803763.1 Zn-dependent hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13552.56 Da        Isoelectric Point: 4.8414

>T58721 WP_035954580.1 NZ_CP013466:c938983-938618 [Burkholderia multivorans]
MVKALFDTNILIDYLSGVESARLELGRYDYRAISTITWMEVLVGTTADDEGPIRAWLSSFDVIPLDSTVADRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGIRVPYQV

Download         Length: 366 bp

>T58721 NZ_CP013466:c938983-938618 [Burkholderia multivorans]
ATGGTAAAAGCTCTCTTCGACACCAACATCCTGATCGACTATCTGAGCGGCGTGGAGTCGGCCCGCCTGGAGCTCGGCCG
CTACGACTACCGTGCGATCAGCACCATTACGTGGATGGAAGTCCTGGTCGGTACGACTGCAGATGACGAAGGCCCGATTC
GCGCGTGGCTCTCGTCGTTCGACGTGATCCCGCTCGACAGTACGGTTGCCGACCGCGCCGTGACCATTCGCAAGGAACGG
CGCATTCGTCTGCCCGACGCGATCGTCTGGGCATCGGCCCAGGTAAACGGGCTGCTGCTCGTGTCGCGTAACACGAAGGA
CTTTCCGGCAACGGAACCCGGCATCCGGGTGCCTTATCAGGTCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8231.38 Da        Isoelectric Point: 4.7640

>AT58721 WP_035956044.1 NZ_CP013466:c939195-938977 [Burkholderia multivorans]
MSRVLVDLSDGQLDELAVIVETEHRPRAAIIRDAIDAYIALHKRPLADDVFGLWKDRAVDGLAYQEKLRSEW

Download         Length: 219 bp

>AT58721 NZ_CP013466:c939195-938977 [Burkholderia multivorans]
ATGAGCCGAGTCTTGGTGGATCTGTCGGACGGCCAGCTCGACGAGCTGGCCGTCATCGTCGAGACTGAGCATCGTCCTCG
CGCCGCGATCATTCGCGATGCGATTGACGCTTACATCGCCCTACACAAGCGCCCGCTCGCCGACGATGTCTTCGGTCTCT
GGAAAGACCGCGCCGTCGACGGGCTCGCTTATCAGGAAAAACTGCGCTCGGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S9ML41

Antitoxin

Source ID Structure
AlphaFold DB A0A2S9ML45

References