Detailed information of TA system TA058718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2188960..2189615
• Replicon: chromosome
• Accession: NZ_CP013464
• Organism: Burkholderia ubonensis strain MSMB1471WGS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WM29_RS25695
• Protein ID: WP_069237986.1
• Coordinates: 2188960..2189379 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A124REA9
• Locus tag: WM29_RS25700
• Protein ID: WP_042588282.1
• Coordinates: 2189376..2189615 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM29_RS25665	2184158..2184958	+	801	WP_069237985.1	AraC family transcriptional regulator	-
WM29_RS25670	2185045..2185977	+	933	WP_059489340.1	DMT family transporter	-
WM29_RS25675	2186167..2186739	+	573	WP_059964264.1	hypothetical protein	-
WM29_RS25680	2186842..2186976	-	135	WP_029226154.1	entericidin A/B family lipoprotein	-
WM29_RS25685	2187193..2188488	+	1296	WP_059963981.1	aspartate carbamoyltransferase	-
WM29_RS25690	2188633..2188950	+	318	WP_059656354.1	hypothetical protein	-
WM29_RS25695	2188960..2189379	-	420	WP_069237986.1	type II toxin-antitoxin system VapC family toxin	Toxin
WM29_RS25700	2189376..2189615	-	240	WP_042588282.1	Arc family DNA-binding protein	Antitoxin
WM29_RS25705	2189925..2192162	+	2238	WP_069237987.1	TonB-dependent siderophore receptor	-
WM29_RS25710	2192166..2192921	+	756	WP_059963987.1	sel1 repeat family protein	-
WM29_RS25715	2192918..2193601	+	684	WP_060306923.1	Fe2+-dependent dioxygenase	-
WM29_RS25720	2193723..2193959	+	237	WP_012366463.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
WM29_RS25725	2193956..2194351	+	396	Protein_1953	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14980.24 Da
• Isoelectric Point: 5.8883

>T58718 WP_069237986.1 NZ_CP013464:c2189379-2188960 [Burkholderia ubonensis]
MILVDTNVLSEPLRPAPNASVLEWLDAQNVETLFLAAISLAELRFGVAAMPEGKRRDWLHRSIEQRVVPAFRGRILPFDA
AASEAYAKLRARARAAGSVFAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAV

Nucleotide

• Length: 420 bp

>T58718 NZ_CP013464:c2189379-2188960 [Burkholderia ubonensis]
ATGATTCTTGTCGATACCAACGTCTTGTCCGAGCCGCTGCGGCCTGCGCCGAATGCGTCAGTGCTGGAGTGGCTCGATGC
GCAGAATGTCGAAACGTTGTTTCTCGCGGCAATCAGTCTGGCCGAGTTGAGATTCGGCGTTGCGGCCATGCCGGAAGGCA
AGCGGCGAGACTGGCTGCATCGAAGCATCGAGCAACGCGTGGTGCCGGCGTTTCGCGGCCGGATCCTGCCGTTCGATGCG
GCTGCGAGCGAGGCTTATGCCAAGCTGCGCGCCCGCGCGCGGGCGGCCGGCAGCGTGTTCGCGCCGGCCGACGGCTATAT
CGCCGCGACCGCCGAAGCCAATGGATTGATCGTGGCGACGCGCGACGTCGCGCCATTTGAAGCAGCCGGCCTGCGGGTGA
TCGATCCGTGGGCGGTGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8840.10 Da
• Isoelectric Point: 5.7135

>AT58718 WP_042588282.1 NZ_CP013464:c2189615-2189376 [Burkholderia ubonensis]
MPVITVRNLPDEVHRALRVRAAQHGRSTEAEVRDILEKAVQPEGRVKLGTLLAEIGREIGGVDLDIQRDKTPTEPMNFE

Nucleotide

• Length: 240 bp

>AT58718 NZ_CP013464:c2189615-2189376 [Burkholderia ubonensis]
ATGCCCGTGATAACCGTTCGGAATTTGCCTGACGAAGTCCATCGCGCCCTTCGGGTTCGCGCGGCGCAGCATGGGCGCAG
CACCGAAGCGGAGGTGCGTGACATCCTCGAGAAAGCGGTTCAGCCGGAAGGGCGCGTCAAGTTGGGAACGTTGCTGGCGG
AAATCGGGCGGGAAATCGGGGGTGTCGATCTCGACATTCAGCGCGACAAAACCCCAACCGAGCCGATGAACTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A124REA9