Detailed information of TA system TA058698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 827712..828244
• Replicon: chromosome
• Accession: NZ_CP013450
• Organism: Burkholderia cenocepacia strain MSMB384WGS

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B4EDC8
• Locus tag: WL90_RS03770
• Protein ID: WP_006484901.1
• Coordinates: 827712..828035 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B4EDC7
• Locus tag: WL90_RS03775
• Protein ID: WP_012492929.1
• Coordinates: 828032..828244 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WL90_RS03740	822731..823564	-	834	WP_034200961.1	class II glutamine amidotransferase	-
WL90_RS03750	823765..824094	-	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
WL90_RS03755	824224..825171	+	948	WP_034200960.1	AraC family transcriptional regulator	-
WL90_RS03760	825275..825718	+	444	WP_011544671.1	YiaA/YiaB family protein	-
WL90_RS35110	825841..826866	-	1026	WP_080435191.1	relaxase/mobilization nuclease domain-containing protein	-
WL90_RS03765	826868..827392	-	525	WP_012492930.1	hypothetical protein	-
WL90_RS03770	827712..828035	-	324	WP_006484901.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
WL90_RS03775	828032..828244	-	213	WP_012492929.1	antitoxin MazE family protein	Antitoxin
WL90_RS03780	828393..828845	-	453	WP_006484947.1	hypothetical protein	-
WL90_RS03785	828913..829308	-	396	WP_195904380.1	hypothetical protein	-
WL90_RS03790	829447..829767	+	321	WP_012492928.1	helix-turn-helix domain-containing protein	-
WL90_RS03795	829942..830349	+	408	WP_069275285.1	transposase	-
WL90_RS03800	830346..830693	+	348	WP_006764055.1	IS66 family insertion sequence element accessory protein TnpB	-
WL90_RS03805	830723..832285	+	1563	WP_060267136.1	IS66 family transposase	-
WL90_RS03810	832326..832922	+	597	WP_060267133.1	plasmid pRiA4b ORF-3 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11652.77 Da
• Isoelectric Point: 8.9366

>T58698 WP_006484901.1 NZ_CP013450:c828035-827712 [Burkholderia cenocepacia]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSAMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T58698 NZ_CP013450:c828035-827712 [Burkholderia cenocepacia]
ATGAATCGCGGGAGCATTGTCACAGTCGCGCTCCAAGGTGACTTCGGCAAGGCTCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTTGCCGAACATCCCAGCGTAACGTTGCTGCTAATGTCGAGCGCAATGGTCGATGCGCCCTTGATACGCATTACTG
TTGTCCCATCCGAGCAGAACGGCTTGCGCATGCCGTCCCAGATTGCCGTAGACAAAATGTTCACCGTGCGCCGCGAGAAA
ATCGGTCGCCCCATCGGCCACCTCGAAGATGAACTGATGGTTGCCGTCAATCGTTCGCTACTCGTTTTTCTAGGACTAGC
GTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8025.99 Da
• Isoelectric Point: 5.5492

>AT58698 WP_012492929.1 NZ_CP013450:c828244-828032 [Burkholderia cenocepacia]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRQPGFADECARQSRMVHQSIDENDLLDFIEQVTDLGHSQ

Nucleotide

• Length: 213 bp

>AT58698 NZ_CP013450:c828244-828032 [Burkholderia cenocepacia]
ATGGCAGCTACCACTTCCGAGCGTGTTCGAATTCACCGCGAGAATCTTCGTGCGGCCGGGCTACGTCCAATTCAGATATG
GGTGCCGGACGTCCGCCAGCCGGGTTTCGCCGATGAATGCGCTCGGCAATCGCGCATGGTCCACCAGAGCATCGACGAGA
ACGATCTGCTCGATTTCATTGAACAGGTTACGGACCTCGGACACTCACAATGA

Structures

Toxin

• AlphaFold DB: B4EDC8

Antitoxin

• AlphaFold DB: B4EDC7