Detailed information of TA system TA058695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2453069..2453724
• Replicon: chromosome
• Accession: NZ_CP013447
• Organism: Burkholderia ubonensis strain MSMB1189WGS isolate MSMB1189WGS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WK67_RS30570
• Protein ID: WP_069271510.1
• Coordinates: 2453069..2453488 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A108D2Q4
• Locus tag: WK67_RS30575
• Protein ID: WP_059635510.1
• Coordinates: 2453485..2453724 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WK67_RS30545	2448590..2449390	+	801	WP_059488890.1	AraC family transcriptional regulator	-
WK67_RS30550	2449477..2450418	+	942	WP_069271901.1	DMT family transporter	-
WK67_RS30555	2450608..2451180	+	573	WP_069271902.1	permease	-
WK67_RS30560	2451296..2452591	+	1296	WP_010089460.1	aspartate carbamoyltransferase	-
WK67_RS30565	2452741..2453058	+	318	WP_059477386.1	hypothetical protein	-
WK67_RS30570	2453069..2453488	-	420	WP_069271510.1	type II toxin-antitoxin system VapC family toxin	Toxin
WK67_RS30575	2453485..2453724	-	240	WP_059635510.1	Arc family DNA-binding protein	Antitoxin
WK67_RS30580	2454034..2456271	+	2238	WP_069271511.1	TonB-dependent siderophore receptor	-
WK67_RS30585	2456275..2457030	+	756	WP_069271512.1	sel1 repeat family protein	-
WK67_RS30590	2457027..2457710	+	684	WP_059952728.1	Fe2+-dependent dioxygenase	-
WK67_RS30595	2457836..2458075	+	240	WP_011659184.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
WK67_RS30600	2458072..2458467	+	396	WP_059801650.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15006.34 Da
• Isoelectric Point: 5.8883

>T58695 WP_069271510.1 NZ_CP013447:c2453488-2453069 [Burkholderia ubonensis]
MILVDTNILSEPLRPAPNVSVLEWLDAQNVETLFLAAISLAEMRFGIAAMPEGKRRDWLHRSIEQRVVPVFRGRILPFDA
AASEAYGKLRARARAAGSVIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAA

Nucleotide

• Length: 420 bp

>T58695 NZ_CP013447:c2453488-2453069 [Burkholderia ubonensis]
ATGATCCTTGTCGATACCAACATCTTGTCCGAGCCGCTGCGGCCTGCGCCGAATGTGTCAGTGCTCGAGTGGCTCGATGC
GCAGAATGTCGAAACGTTATTTCTCGCGGCAATTAGTCTGGCCGAGATGAGATTCGGCATCGCGGCCATGCCGGAAGGCA
AGCGGCGAGACTGGCTGCATCGCAGCATCGAACAACGCGTGGTGCCGGTGTTTCGCGGCCGGATTCTGCCGTTCGACGCG
GCTGCGAGCGAGGCCTATGGCAAGCTTCGCGCCCGCGCGCGGGCGGCCGGCAGCGTGATCGCGCCGGCCGACGGCTATAT
CGCCGCGACTGCCGAAGCAAATGGATTGATCGTGGCGACGCGCGACGTCGCGCCGTTTGAAGCGGCCGGCCTGCGGGTGA
TCGATCCGTGGGCGGCCTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8810.07 Da
• Isoelectric Point: 5.7135

>AT58695 WP_059635510.1 NZ_CP013447:c2453724-2453485 [Burkholderia ubonensis]
MPVITVRNLPDEVHRALRVRAAQHGRSTEAEVRDILEKAVQPEGRVKLGTLLAEIGREIGGVDLDIQRDKTPAEPMNFE

Nucleotide

• Length: 240 bp

>AT58695 NZ_CP013447:c2453724-2453485 [Burkholderia ubonensis]
ATGCCCGTGATAACCGTTCGGAATTTGCCTGACGAAGTCCATCGCGCCCTTCGCGTTCGCGCGGCGCAGCACGGACGCAG
CACCGAGGCGGAGGTGCGTGACATCCTCGAGAAAGCGGTTCAGCCGGAAGGGCGCGTCAAATTGGGAACGTTGCTGGCGG
AAATCGGGCGGGAAATCGGGGGTGTCGATCTCGACATTCAGCGCGACAAAACCCCAGCCGAGCCGATGAACTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A108D2Q4