Detailed information of TA system TA058686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 177619..178256
• Replicon: chromosome
• Accession: NZ_CP013446
• Organism: Burkholderia ubonensis strain MSMB1189WGS isolate MSMB1189WGS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WK67_RS04050
• Protein ID: WP_059959927.1
• Coordinates: 177855..178256 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A103VAC2
• Locus tag: WK67_RS04045
• Protein ID: WP_059586337.1
• Coordinates: 177619..177855 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WK67_RS04025	173612..174661	-	1050	WP_069269770.1	ADP-heptose--LPS heptosyltransferase	-
WK67_RS04030	174678..176381	-	1704	WP_069269771.1	thiamine pyrophosphate-binding protein	-
WK67_RS04035	176650..177108	+	459	WP_059983870.1	hypothetical protein	-
WK67_RS04040	177105..177413	+	309	WP_069269772.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
WK67_RS04045	177619..177855	+	237	WP_059586337.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
WK67_RS04050	177855..178256	+	402	WP_059959927.1	type II toxin-antitoxin system VapC family toxin	Toxin
WK67_RS04055	178301..179689	-	1389	WP_059717597.1	L-serine ammonia-lyase	-
WK67_RS04060	179718..180860	-	1143	WP_059586483.1	alginate lyase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-120)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15141.30 Da
• Isoelectric Point: 4.9366

>T58686 WP_059959927.1 NZ_CP013446:177855-178256 [Burkholderia ubonensis]
MPRYMLDTNMCIYLMKNQPEQVAKRFARYYVGDVVMSAITYAELEYGVAVCANPDRERRNLAALIEDIPVAPFDVAAARA
YGPVREATRERKNDQLDKLIAAHAISLDVVLVTNNERDFASYPAVRLENWLND

Nucleotide

• Length: 402 bp

>T58686 NZ_CP013446:177855-178256 [Burkholderia ubonensis]
ATGCCGCGTTACATGCTCGACACCAACATGTGCATCTACCTGATGAAGAACCAGCCGGAGCAGGTTGCGAAACGCTTTGC
GCGGTACTACGTCGGTGACGTGGTGATGTCGGCGATCACCTATGCCGAGCTCGAGTATGGTGTCGCGGTCTGCGCGAATC
CTGATCGCGAGCGCCGAAATCTCGCGGCGCTGATCGAGGACATTCCCGTCGCCCCGTTCGATGTGGCGGCGGCGCGGGCC
TACGGGCCGGTGCGGGAGGCAACGCGGGAGCGCAAGAATGACCAGCTGGACAAGCTGATCGCCGCGCATGCGATTTCGCT
TGACGTTGTACTCGTGACCAACAACGAGCGGGATTTTGCCAGCTATCCCGCCGTGAGGCTGGAGAACTGGCTGAACGATT
AG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8864.94 Da
• Isoelectric Point: 5.7317

>AT58686 WP_059586337.1 NZ_CP013446:177619-177855 [Burkholderia ubonensis]
MHTTRAFRNGNSQAVRIPADLAFERSDIELEIERVGDEIRIRPARRPLAGVLEKFAKFGPDFMSDGRGEHEQAEREGL

Nucleotide

• Length: 237 bp

>AT58686 NZ_CP013446:177619-177855 [Burkholderia ubonensis]
ATGCATACCACCCGAGCGTTCAGGAACGGCAACTCCCAAGCTGTGCGTATTCCTGCGGACCTCGCTTTCGAGCGCAGCGA
CATCGAACTTGAAATCGAGCGAGTGGGCGATGAAATTCGCATTCGACCTGCTCGGCGGCCGTTGGCTGGCGTGCTCGAGA
AGTTCGCGAAATTCGGGCCGGATTTCATGTCCGACGGACGTGGCGAGCACGAGCAGGCCGAACGCGAGGGTCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A103VAC2