Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 2435454..2436109 Replicon chromosome
Accession NZ_CP013444
Organism Burkholderia cepacia strain MSMB1184WGS

Toxin (Protein)


Gene name vapC Uniprot ID A0A1B4Q1V9
Locus tag WT26_RS30705 Protein ID WP_069274705.1
Coordinates 2435454..2435873 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1B4Q1W5
Locus tag WT26_RS30710 Protein ID WP_069274706.1
Coordinates 2435870..2436109 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WT26_RS30680 2430976..2431776 + 801 WP_069274703.1 AraC family transcriptional regulator -
WT26_RS30685 2431863..2432804 + 942 WP_069275202.1 DMT family transporter -
WT26_RS30690 2432994..2433566 + 573 WP_069275203.1 permease -
WT26_RS30695 2433681..2434976 + 1296 WP_069274704.1 aspartate carbamoyltransferase -
WT26_RS30700 2435126..2435443 + 318 WP_059477386.1 hypothetical protein -
WT26_RS30705 2435454..2435873 - 420 WP_069274705.1 type II toxin-antitoxin system VapC family toxin Toxin
WT26_RS30710 2435870..2436109 - 240 WP_069274706.1 Arc family DNA-binding protein Antitoxin
WT26_RS30715 2436419..2438656 + 2238 WP_069274707.1 TonB-dependent siderophore receptor -
WT26_RS30720 2438660..2439427 + 768 WP_059525720.1 sel1 repeat family protein -
WT26_RS30725 2439424..2440107 + 684 WP_059525718.1 Fe2+-dependent dioxygenase -
WT26_RS30730 2440199..2440438 + 240 WP_011659184.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
WT26_RS30735 2440435..2440830 + 396 WP_059663276.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-122)

Antitoxin

Arc-like DNA binding domain

(7-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14992.31 Da        Isoelectric Point: 5.8883

>T58683 WP_069274705.1 NZ_CP013444:c2435873-2435454 [Burkholderia cepacia]
MILVDTNVISEPLRPAPNVSVLEWLDAQNVETLFLAAISLAEMRFGIAAMPEGKRRDWLHRSIEQRVVPVFRGRILPFDA
AASEAYGKLRARARAAGSVIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAA

Download         Length: 420 bp

>T58683 NZ_CP013444:c2435873-2435454 [Burkholderia cepacia]
ATGATCCTTGTCGATACGAACGTCATATCCGAACCGCTGCGGCCTGCGCCGAATGTGTCAGTGCTCGAGTGGCTCGATGC
GCAGAATGTCGAAACGTTATTTCTCGCGGCAATTAGTCTGGCCGAGATGAGATTCGGCATCGCGGCCATGCCGGAAGGCA
AGCGGCGAGACTGGCTGCATCGCAGCATCGAACAACGCGTGGTGCCGGTGTTTCGCGGCCGGATTCTGCCGTTCGACGCG
GCTGCGAGCGAGGCCTATGGCAAGCTTCGCGCCCGCGCGCGGGCGGCCGGCAGCGTGATCGCGCCGGCCGACGGCTATAT
CGCCGCGACTGCCGAAGCAAATGGATTGATCGTGGCGACGCGCGACGTCGCGCCGTTTGAAGCGGCCGGCCTGCGGGTGA
TCGATCCGTGGGCGGCCTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8753.02 Da        Isoelectric Point: 5.7066

>AT58683 WP_069274706.1 NZ_CP013444:c2436109-2435870 [Burkholderia cepacia]
MPVITVRNLPDEVHRALRIRAAQHGRSTEAEVRDILEKAVQPEGRVKLGTLLAEIGREIGGVDLDIQRDKTPADPMGFE

Download         Length: 240 bp

>AT58683 NZ_CP013444:c2436109-2435870 [Burkholderia cepacia]
ATGCCGGTGATCACCGTTCGAAATTTGCCTGACGAAGTGCACCGCGCACTTCGGATTCGCGCGGCCCAGCACGGACGCAG
CACCGAAGCCGAGGTGCGTGACATTCTCGAGAAAGCGGTTCAGCCGGAAGGGCGCGTCAAATTGGGAACGTTGTTGGCGG
AAATCGGGCGGGAAATCGGGGGTGTCGATCTCGACATTCAGCGCGACAAGACGCCAGCCGATCCGATGGGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B4Q1V9

Antitoxin

Source ID Structure
AlphaFold DB A0A1B4Q1W5

References