Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF-MazE
Location 842658..843220 Replicon chromosome
Accession NZ_CP013441
Organism Burkholderia vietnamiensis strain HI2297

Toxin (Protein)


Gene name mazE Uniprot ID A0A1B4LIN8
Locus tag WK31_RS27975 Protein ID WP_011879712.1
Coordinates 842658..842978 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name mazF Uniprot ID A0A1B4LIX1
Locus tag WK31_RS27980 Protein ID WP_045579168.1
Coordinates 842972..843220 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WK31_RS27950 838159..838881 - 723 WP_069246641.1 ABC transporter permease subunit -
WK31_RS27955 838974..839834 - 861 WP_045579531.1 transporter substrate-binding domain-containing protein -
WK31_RS27960 840144..840347 - 204 Protein_750 YhcG family protein -
WK31_RS31940 840518..840709 - 192 WP_124259719.1 hypothetical protein -
WK31_RS27965 840794..840979 - 186 WP_043292729.1 hypothetical protein -
WK31_RS27970 841377..841913 - 537 WP_155121940.1 hypothetical protein -
WK31_RS31945 841885..842130 - 246 WP_131754457.1 hypothetical protein -
WK31_RS27975 842658..842978 - 321 WP_011879712.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
WK31_RS27980 842972..843220 - 249 WP_045579168.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
WK31_RS27990 843428..844617 + 1190 WP_155744937.1 IS3 family transposase -
WK31_RS27995 844765..845874 - 1110 WP_011879708.1 NAD(P)/FAD-dependent oxidoreductase -
WK31_RS28000 845893..846304 - 412 Protein_759 DUF2703 domain-containing protein -
WK31_RS28005 846325..846725 - 401 Protein_760 arsenical pump-driving ATPase -
WK31_RS31140 846817..846987 - 171 WP_080484375.1 hypothetical protein -
WK31_RS31145 847024..847467 - 444 WP_043292565.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 840794..874639 33845


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-104)

Antitoxin

SpoVT-AbrB domain

(9-44)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 11935.23 Da        Isoelectric Point: 10.5568

>T58673 WP_011879712.1 NZ_CP013441:c842978-842658 [Burkholderia vietnamiensis]
MVMRGEVWLVALDPTLGSEIQKTRPCVVVSPPEMHDHLRTVIVAPMTSKGRPVPFRIPVTFKRKHGLILLDQIRAVDKVR
LVKKEGAVADKTLLDTLRTLQEVFAE

Download         Length: 321 bp

>T58673 NZ_CP013441:c842978-842658 [Burkholderia vietnamiensis]
ATGGTGATGCGCGGGGAAGTCTGGTTGGTCGCACTCGATCCGACGCTTGGTAGCGAGATTCAAAAGACGCGCCCCTGTGT
GGTCGTTTCACCTCCCGAAATGCACGACCACTTGCGCACTGTCATCGTCGCGCCGATGACTTCCAAGGGGCGTCCAGTGC
CGTTTCGGATTCCGGTTACGTTCAAGCGCAAACATGGTTTGATCCTTCTGGATCAGATTCGCGCAGTGGACAAGGTGCGC
TTGGTTAAGAAAGAGGGTGCAGTTGCCGATAAAACGTTGTTGGATACCCTGCGGACCTTGCAGGAAGTCTTTGCGGAATA
A

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8979.19 Da        Isoelectric Point: 4.4929

>AT58673 WP_045579168.1 NZ_CP013441:c843220-842972 [Burkholderia vietnamiensis]
MKTTIRRMGNSQGVLIPKPVLAQLGLESEVEMEVENDAIVLRRPKHKAREGWAEASKALAESGDDALVMGEFSNADDAEL
AW

Download         Length: 249 bp

>AT58673 NZ_CP013441:c843220-842972 [Burkholderia vietnamiensis]
ATGAAGACGACGATTCGCAGAATGGGTAACTCGCAGGGCGTGCTGATTCCGAAGCCCGTCCTTGCGCAACTCGGGCTGGA
GAGCGAAGTGGAAATGGAGGTCGAGAACGACGCGATTGTTCTGCGCCGCCCGAAGCACAAAGCCCGTGAGGGGTGGGCGG
AAGCAAGCAAGGCTCTGGCCGAGAGCGGCGACGATGCGCTGGTTATGGGCGAGTTTTCGAACGCCGATGACGCGGAGCTT
GCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B4LIN8

Antitoxin

Source ID Structure
AlphaFold DB A0A1B4LIX1

References