Detailed information of TA system TA058665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 34565..35214
• Replicon: plasmid pAU17928
• Accession: NZ_CP013436
• Organism: Burkholderia latens strain AU17928 isolate AU17928

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WK25_RS15660
• Protein ID: WP_011695140.1
• Coordinates: 34565..34975 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A103EQ81
• Locus tag: WK25_RS15665
• Protein ID: WP_059569609.1
• Coordinates: 34972..35214 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WK25_RS15630	30600..31577	+	978	WP_069242035.1	IS5 family transposase	-
WK25_RS15635	31899..32348	-	450	WP_069242036.1	hypothetical protein	-
WK25_RS15640	32361..32774	-	414	WP_069242037.1	DUF2442 domain-containing protein	-
WK25_RS15645	32777..33235	-	459	WP_156789043.1	hypothetical protein	-
WK25_RS15650	33658..33858	-	201	WP_069242039.1	hypothetical protein	-
WK25_RS15655	33907..34254	-	348	WP_069242040.1	hypothetical protein	-
WK25_RS15660	34565..34975	-	411	WP_011695140.1	type II toxin-antitoxin system VapC family toxin	Toxin
WK25_RS15665	34972..35214	-	243	WP_059569609.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
WK25_RS15670	35336..35764	-	429	Protein_43	DNA ligase	-
WK25_RS15675	35818..36795	-	978	WP_069242041.1	IS5 family transposase	-
WK25_RS15680	36857..37057	+	201	Protein_45	IS4 family transposase	-
WK25_RS31275	37970..38887	+	918	WP_156789044.1	hypothetical protein	-
WK25_RS15685	38980..39957	-	978	WP_069242019.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..45481	45481

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14773.00 Da
• Isoelectric Point: 5.1526

>T58665 WP_011695140.1 NZ_CP013436:c34975-34565 [Burkholderia latens]
MTLYLLDTNILSNVIRDPRGACATRIGETPPEQVCTSIVVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIGANDMLIAAHALAVDAVLVTDNTAEFTRVAGLPVENWLRSAT

Nucleotide

• Length: 411 bp

>T58665 NZ_CP013436:c34975-34565 [Burkholderia latens]
ATGACGCTGTACCTCCTCGATACCAACATTTTGTCGAACGTGATCCGCGACCCGCGCGGCGCATGCGCCACCCGGATCGG
AGAAACCCCGCCGGAGCAAGTGTGCACCTCGATCGTCGTCGCGGCGGAACTGCGGTTCGGCGTCTGGAAGCGCGGCTCGT
CAACGCTCGCACAGCGCGTCGAGCAGCTGCTCGCGAGCCTGACCGTGTTGCCGCTGCAGCCCGACGCGGACCGGTGCTAC
GGGCGGTTGCGGGCCGAGCTCGAGAAGCAAGGGCAGCTGATCGGCGCCAACGACATGCTGATCGCCGCGCATGCGCTGGC
CGTCGACGCGGTCCTGGTCACAGACAACACGGCTGAGTTCACCCGCGTTGCCGGGCTGCCGGTCGAAAACTGGCTGCGAT
CGGCGACGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9350.59 Da
• Isoelectric Point: 4.6856

>AT58665 WP_059569609.1 NZ_CP013436:c35214-34972 [Burkholderia latens]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWTPLDETFPDIEDLPPEPVDIE

Nucleotide

• Length: 243 bp

>AT58665 NZ_CP013436:c35214-34972 [Burkholderia latens]
ATGCCAACCCATGACACCCGCCACGCGCGCCTGTTTCGCAACGGGCGAAGCCAAGCCGTGCGGATTCCGCGCGAATTCGA
ATTGCCGGTCTCGGAGGTCACCATCCACCGGGAAGGCCGGCGCCTGATCATCGAGCCCGTAGAGGCCGCGCCCTTGCGCG
AGCTGTTCGCCCAATGGACCCCGCTGGACGAGACCTTTCCGGACATCGAGGACCTTCCGCCTGAACCGGTCGACATCGAA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A103EQ81