Detailed information of TA system TA058664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 10955..11604
• Replicon: plasmid pAU17928
• Accession: NZ_CP013436
• Organism: Burkholderia latens strain AU17928 isolate AU17928

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WK25_RS15515
• Protein ID: WP_011695140.1
• Coordinates: 11194..11604 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A103EQ81
• Locus tag: WK25_RS15510
• Protein ID: WP_059569609.1
• Coordinates: 10955..11197 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WK25_RS15500	6305..7531	-	1227	WP_069242022.1	replication initiation protein	-
WK25_RS15505	8768..9394	-	627	WP_069242023.1	hypothetical protein	-
WK25_RS30495	9391..9747	-	357	WP_083253022.1	hypothetical protein	-
WK25_RS15510	10955..11197	+	243	WP_059569609.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
WK25_RS15515	11194..11604	+	411	WP_011695140.1	type II toxin-antitoxin system VapC family toxin	Toxin
WK25_RS15520	11916..12263	+	348	WP_069242024.1	hypothetical protein	-
WK25_RS15525	12311..12511	+	201	WP_069242025.1	hypothetical protein	-
WK25_RS15530	12701..13414	+	714	WP_083253023.1	helix-turn-helix domain-containing protein	-
WK25_RS31260	13404..13610	+	207	WP_156789038.1	hypothetical protein	-
WK25_RS15535	13641..13874	+	234	WP_156789039.1	hypothetical protein	-
WK25_RS31265	13923..14216	+	294	WP_156789040.1	hypothetical protein	-
WK25_RS15540	14486..14929	+	444	WP_069242027.1	hypothetical protein	-
WK25_RS15545	15023..15826	+	804	WP_156789041.1	HNH endonuclease	-
WK25_RS15550	15905..16522	+	618	WP_069242029.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..45481	45481

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14773.00 Da
• Isoelectric Point: 5.1526

>T58664 WP_011695140.1 NZ_CP013436:11194-11604 [Burkholderia latens]
MTLYLLDTNILSNVIRDPRGACATRIGETPPEQVCTSIVVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIGANDMLIAAHALAVDAVLVTDNTAEFTRVAGLPVENWLRSAT

Nucleotide

• Length: 411 bp

>T58664 NZ_CP013436:11194-11604 [Burkholderia latens]
ATGACGCTGTACCTGCTCGATACCAACATTTTGTCGAACGTGATCCGCGACCCGCGCGGCGCATGCGCCACCCGGATCGG
AGAGACCCCGCCGGAGCAAGTGTGCACCTCGATCGTCGTCGCCGCGGAACTGCGGTTCGGCGTCTGGAAGCGCGGCTCGT
CGACGCTCGCGCAGCGCGTCGAGCAACTGCTCGCGAGCCTGACCGTGTTGCCGCTGCAGCCCGACGCGGACCGCTGCTAC
GGGCGGTTGCGGGCCGAGCTCGAGAAGCAAGGCCAGCTGATCGGCGCCAACGACATGCTGATCGCCGCGCACGCGCTGGC
CGTCGACGCCGTGCTGGTCACGGACAACACGGCTGAATTCACCCGCGTTGCCGGGCTGCCGGTCGAAAACTGGCTGCGAT
CGGCGACATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9350.59 Da
• Isoelectric Point: 4.6856

>AT58664 WP_059569609.1 NZ_CP013436:10955-11197 [Burkholderia latens]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWTPLDETFPDIEDLPPEPVDIE

Nucleotide

• Length: 243 bp

>AT58664 NZ_CP013436:10955-11197 [Burkholderia latens]
ATGCCAACCCATGACACCCGCCACGCGCGCCTGTTTCGCAACGGGCGCAGCCAGGCCGTGCGGATCCCGCGCGAATTCGA
ATTACCGGTCTCGGAGGTCACCATCCACCGGGAAGGCCGCCGCTTGATCATCGAGCCCGTAGAGGCCGCGCCCTTGCGCG
AGCTGTTCGCCCAATGGACCCCGCTGGACGAGACGTTTCCGGACATCGAGGACCTTCCGCCTGAACCGGTCGACATCGAA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A103EQ81