Detailed information of TA system TA058660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 220418..220980
• Replicon: chromosome
• Accession: NZ_CP013434
• Organism: Burkholderia vietnamiensis strain AU1233

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: WK23_RS25545
• Protein ID: WP_069264977.1
• Coordinates: 220418..220738 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: WK23_RS25550
• Protein ID: WP_069264979.1
• Coordinates: 220732..220980 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WK23_RS32610	215614..215778	+	165	WP_155625324.1	hypothetical protein	-
WK23_RS25515	215802..216290	+	489	Protein_162	DDE-type integrase/transposase/recombinase	-
WK23_RS25520	216540..217583	+	1044	WP_069224830.1	DUF1016 family protein	-
WK23_RS31430	218019..218333	-	315	WP_172820468.1	H-NS histone family protein	-
WK23_RS25530	218611..219072	-	462	WP_126221127.1	hypothetical protein	-
WK23_RS32615	219120..219566	-	447	WP_126221126.1	hypothetical protein	-
WK23_RS32620	219580..219831	-	252	WP_155625382.1	hypothetical protein	-
WK23_RS25540	219865..220089	-	225	WP_069264976.1	hypothetical protein	-
WK23_RS31440	220131..220406	-	276	WP_081333285.1	YkgJ family cysteine cluster protein	-
WK23_RS25545	220418..220738	-	321	WP_069264977.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
WK23_RS25550	220732..220980	-	249	WP_069264979.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
WK23_RS25555	221107..221778	-	672	WP_069264981.1	site-specific integrase	-
WK23_RS25560	221942..222268	+	327	WP_045579170.1	H-NS histone family protein	-
WK23_RS25565	222399..223310	+	912	WP_069264983.1	SOS response-associated peptidase family protein	-
WK23_RS32625	223872..224213	-	342	WP_155772662.1	hypothetical protein	-
WK23_RS25575	224446..225447	-	1002	WP_059896631.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	180470..232462	51992

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-103)

Antitoxin

• SpoVT-AbrB domain (9-44)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11926.22 Da
• Isoelectric Point: 10.5567

>T58660 WP_069264977.1 NZ_CP013434:c220738-220418 [Burkholderia vietnamiensis]
MVMRGEVWLVALDPTLGSEIQKTRPCVVVSPPEMHDHLRTVIVAPMTSKGRPAPFRIPVTFKRKQGLILLDQIRAVDKVR
LVKKEGVVADKTLLDTLRTLQEVFAE

Nucleotide

• Length: 321 bp

>T58660 NZ_CP013434:c220738-220418 [Burkholderia vietnamiensis]
ATGGTGATGCGCGGGGAGGTCTGGTTGGTCGCACTCGATCCGACGCTTGGTAGCGAGATTCAAAAGACGCGCCCCTGTGT
GGTCGTTTCGCCTCCCGAAATGCACGATCACTTGCGCACAGTCATCGTCGCGCCGATGACTTCAAAGGGGCGTCCTGCAC
CGTTTCGGATTCCAGTTACGTTCAAGCGCAAACAGGGCTTGATCCTCCTGGACCAGATTCGCGCCGTAGACAAGGTGCGC
CTGGTAAAGAAAGAGGGCGTAGTCGCCGATAAGACGTTGTTGGATACCCTGCGAACCTTGCAGGAAGTCTTTGCAGAATA
G

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9007.20 Da
• Isoelectric Point: 4.4929

>AT58660 WP_069264979.1 NZ_CP013434:c220980-220732 [Burkholderia vietnamiensis]
MRTTIRRMGNSQGVLIPKPVLAQLGLESEVEMEVENDAIVLRRPKHKAREGWAEASKALAESGDDALVMGEFSNADDAEL
AW

Nucleotide

• Length: 249 bp

>AT58660 NZ_CP013434:c220980-220732 [Burkholderia vietnamiensis]
ATGAGAACGACGATTCGCAGAATGGGTAACTCGCAGGGCGTGCTGATTCCGAAGCCCGTCCTTGCGCAACTCGGGCTGGA
GAGCGAAGTGGAAATGGAGGTCGAGAACGACGCGATTGTTCTGCGCCGCCCGAAGCACAAAGCCCGTGAGGGTTGGGCGG
AAGCAAGCAAGGCTCTGGCGGAGAGCGGCGACGATGCGTTGGTTATGGGCGAGTTTTCGAACGCCGATGACGCGGAGCTT
GCATGGTGA