Detailed information of TA system TA058652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 895531..896186
• Replicon: chromosome
• Accession: NZ_CP013431
• Organism: Burkholderia multivorans strain AU1185 isolate AU1185

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B9BGY2
• Locus tag: WK22_RS20075
• Protein ID: WP_006403072.1
• Coordinates: 895767..896186 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BGX6
• Locus tag: WK22_RS20070
• Protein ID: WP_006403071.1
• Coordinates: 895531..895770 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WK22_RS32155	890593..890736	-	144	WP_006409932.1	hypothetical protein	-
WK22_RS20050	891325..891528	+	204	WP_006403067.1	hypothetical protein	-
WK22_RS20055	891542..892225	-	684	WP_069220955.1	Fe2+-dependent dioxygenase	-
WK22_RS20060	892222..892977	-	756	WP_006403069.1	sel1 repeat family protein	-
WK22_RS20065	892981..895227	-	2247	WP_006403070.1	TonB-dependent siderophore receptor	-
WK22_RS20070	895531..895770	+	240	WP_006403071.1	Arc family DNA-binding protein	Antitoxin
WK22_RS20075	895767..896186	+	420	WP_006403072.1	type II toxin-antitoxin system VapC family toxin	Toxin
WK22_RS20080	896320..897615	-	1296	WP_069220956.1	aspartate carbamoyltransferase	-
WK22_RS20085	897848..897982	+	135	WP_006409923.1	entericidin A/B family lipoprotein	-
WK22_RS20090	898076..898651	-	576	WP_069220957.1	permease	-
WK22_RS20095	898855..899805	-	951	WP_081336848.1	DMT family transporter	-
WK22_RS20100	900072..900866	-	795	WP_059938960.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15086.28 Da
• Isoelectric Point: 5.2157

>T58652 WP_006403072.1 NZ_CP013431:895767-896186 [Burkholderia multivorans]
MILVDTNVISEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLEQSIEHRVVPVFRGRILPFDD
AAGKAYARIRAHARAVGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAS

Nucleotide

• Length: 420 bp

>T58652 NZ_CP013431:895767-896186 [Burkholderia multivorans]
ATGATCCTTGTCGATACGAACGTCATTTCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
GCAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTGGAGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGGGCAAAGCCTATGCGCGCATTCGTGCGCACGCCCGTGCCGTCGGCAACGCGATCGCGCCGGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGTGGGCCTCATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8698.91 Da
• Isoelectric Point: 5.1133

>AT58652 WP_006403071.1 NZ_CP013431:895531-895770 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSTEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTPTDPISFE

Nucleotide

• Length: 240 bp

>AT58652 NZ_CP013431:895531-895770 [Burkholderia multivorans]
ATGCCTGTGATTACCGTTCGAAATTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCGCTGCACGGGCGCAG
TACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGCGTGAAGCTGGGAACCTTACTGGCGG
AAATCGGGCGGGAGGCAGGCGGTGTCGATCTCGACATCCAGCGTGACAAGACGCCAACCGATCCGATCAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: B9BGY2

Antitoxin

• AlphaFold DB: B9BGX6