Detailed information of TA system TA058649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 1059682..1060364
• Replicon: chromosome
• Accession: NZ_CP013430
• Organism: Burkholderia multivorans strain AU1185 isolate AU1185

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: WK22_RS04820
• Protein ID: WP_069220063.1
• Coordinates: 1060005..1060364 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: B9BQB8
• Locus tag: WK22_RS04815
• Protein ID: WP_006405635.1
• Coordinates: 1059682..1060005 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WK22_RS04795	1055031..1056491	+	1461	WP_006405639.1	PLP-dependent aminotransferase family protein	-
WK22_RS04800	1056650..1058548	+	1899	WP_069220062.1	molecular chaperone HtpG	-
WK22_RS04805	1058557..1059171	+	615	WP_006398690.1	chorismate lyase	-
WK22_RS04810	1059168..1059659	+	492	WP_053296985.1	DNA-deoxyinosine glycosylase	-
WK22_RS04815	1059682..1060005	-	324	WP_006405635.1	helix-turn-helix domain-containing protein	Antitoxin
WK22_RS04820	1060005..1060364	-	360	WP_069220063.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WK22_RS04825	1060912..1061166	-	255	WP_059940739.1	hypothetical protein	-
WK22_RS04830	1061395..1062312	+	918	WP_053296988.1	spermidine synthase	-
WK22_RS04835	1062509..1062742	+	234	WP_006398697.1	DUF3311 domain-containing protein	-
WK22_RS04840	1062739..1064289	+	1551	WP_006398698.1	sodium:solute symporter family protein	-
WK22_RS04845	1064393..1064839	-	447	WP_006405630.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 12917.82 Da
• Isoelectric Point: 9.3081

>T58649 WP_069220063.1 NZ_CP013430:c1060364-1060005 [Burkholderia multivorans]
MVPYTKPLYWLGSARKDLKAMPEAVQDTFGYALYLAQTGGKHDQAKPLRGFGSAGVLEVVESEDSGTYRAVYTVKLGGAV
YVLHCFQKKSCRGIATPKPDIELVRARLSAAEAHANGEK

Nucleotide

• Length: 360 bp

>T58649 NZ_CP013430:c1060364-1060005 [Burkholderia multivorans]
ATGGTTCCGTACACCAAACCGCTCTACTGGCTCGGCTCGGCACGCAAGGACTTGAAAGCGATGCCCGAAGCGGTACAGGA
CACGTTCGGCTATGCGCTGTATCTGGCCCAGACGGGAGGAAAGCACGATCAGGCCAAGCCGTTGAGGGGTTTCGGATCGG
CCGGCGTACTCGAGGTCGTGGAGTCGGAGGACAGCGGCACCTACCGGGCGGTCTATACGGTGAAACTCGGTGGCGCCGTC
TATGTGCTGCACTGTTTCCAGAAGAAATCGTGCCGCGGTATCGCTACGCCGAAGCCGGACATCGAACTGGTTCGCGCGCG
CCTGAGCGCCGCCGAAGCACACGCAAATGGAGAAAAGTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11828.69 Da
• Isoelectric Point: 8.0686

>AT58649 WP_006405635.1 NZ_CP013430:c1060005-1059682 [Burkholderia multivorans]
MIEIERGSGNVYADLGTPDAEEMRVKAQLAAKIAEIIKTRKWTQQHAARLLGMTQPKLSNMLRGQFRGVSEAKMLDCLAK
LGRDVQIVVGPDRHSETEGHIEVVFAA

Nucleotide

• Length: 324 bp

>AT58649 NZ_CP013430:c1060005-1059682 [Burkholderia multivorans]
ATGATCGAAATCGAACGGGGCAGCGGGAACGTCTACGCGGATCTTGGCACGCCCGACGCCGAAGAAATGCGCGTCAAGGC
TCAACTCGCCGCCAAGATTGCGGAGATCATCAAGACTCGCAAATGGACGCAGCAGCACGCAGCACGATTGCTCGGCATGA
CGCAACCGAAGCTATCGAACATGCTGCGCGGGCAGTTTCGCGGCGTAAGCGAAGCGAAGATGCTCGACTGTCTCGCCAAG
CTCGGGCGCGATGTACAGATCGTCGTAGGACCGGACCGTCATTCGGAAACCGAAGGACACATCGAGGTCGTGTTCGCTGC
CTGA

Structures

Antitoxin

• AlphaFold DB: B9BQB8