Detailed information of TA system TA058642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 493574..494215
• Replicon: chromosome
• Accession: NZ_CP013428
• Organism: Burkholderia sp. MSMB0856

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A102EQS9
• Locus tag: WS87_RS19855
• Protein ID: WP_059531689.1
• Coordinates: 493877..494215 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: WS87_RS19850
• Protein ID: WP_006750245.1
• Coordinates: 493574..493873 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WS87_RS19825	488747..490018	+	1272	WP_059448779.1	Rieske 2Fe-2S domain-containing protein	-
WS87_RS19830	490036..490521	+	486	WP_059818717.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
WS87_RS19835	490538..490864	+	327	WP_039344765.1	non-heme iron oxygenase ferredoxin subunit	-
WS87_RS19840	490854..492074	+	1221	WP_059818718.1	FAD-dependent oxidoreductase	-
WS87_RS19845	492239..493525	+	1287	WP_059531692.1	DUF445 domain-containing protein	-
WS87_RS19850	493574..493873	-	300	WP_006750245.1	putative addiction module antidote protein	Antitoxin
WS87_RS19855	493877..494215	-	339	WP_059531689.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WS87_RS19865	494572..496212	+	1641	WP_059818719.1	acetolactate synthase large subunit	-
WS87_RS19870	496241..497674	+	1434	WP_059818720.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (16-109)

Antitoxin

• Uncharacterized protein HI1420 (5-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12799.66 Da
• Isoelectric Point: 9.9808

>T58642 WP_059531689.1 NZ_CP013428:c494215-493877 [Burkholderia sp. MSMB0856]
MVDIPRSMSYSAPTFSIRTTDVFDAWFARLQDRVARRRIQARIDRLSMGNPGDWKSAGSPVVEMRVDHGPGYRVYYVRRG
TIWVILLCGGDKSTQQADIRAAHAMLERLDLE

Nucleotide

• Length: 339 bp

>T58642 NZ_CP013428:c494215-493877 [Burkholderia sp. MSMB0856]
ATGGTTGACATACCGCGATCCATGTCCTACAGTGCGCCTACATTCAGTATCCGGACTACCGACGTCTTCGACGCGTGGTT
TGCCCGCCTGCAGGATCGCGTCGCCCGGCGGCGCATCCAGGCGCGCATCGACCGTCTGTCGATGGGCAACCCGGGCGACT
GGAAATCCGCCGGCTCACCCGTCGTCGAAATGCGTGTCGACCACGGCCCCGGCTATCGCGTCTATTACGTCAGACGCGGA
ACGATATGGGTGATCCTGCTCTGCGGCGGCGACAAGTCGACGCAACAGGCGGACATCCGCGCCGCGCACGCGATGCTCGA
GCGGCTCGACCTGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10674.11 Da
• Isoelectric Point: 6.6384

>AT58642 WP_006750245.1 NZ_CP013428:c493873-493574 [Burkholderia sp. MSMB0856]
MDKIKTRPWDSAEHLKTDDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT58642 NZ_CP013428:c493873-493574 [Burkholderia sp. MSMB0856]
ATGGACAAGATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGACGACGACATGGCCGACTACTTCGAGGC
CTGTCTGCAGGAAGCCGGCGACGACCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGCGCGCGCGGCATGTCGC
AGGTCGCGCGCGATGCGGGCCTGTCGCGCGAAGGTCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTGATCAAGGCCCTCGGCCTGCAACTGCACGGCGCGAAGGCACACGCTTGA

Structures

Toxin

• AlphaFold DB: A0A102EQS9