Detailed information of TA system TA058631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 241314..241972
• Replicon: chromosome
• Accession: NZ_CP013421
• Organism: Burkholderia ubonensis strain MSMB0783

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WJ35_RS16175
• Protein ID: WP_043292779.1
• Coordinates: 241541..241972 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1B4LJE8
• Locus tag: WJ35_RS16170
• Protein ID: WP_043292778.1
• Coordinates: 241314..241541 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WJ35_RS30250	237149..237857	-	709	Protein_261	cupin domain-containing protein	-
WJ35_RS16155	238043..238450	-	408	WP_011880193.1	DUF934 domain-containing protein	-
WJ35_RS16160	238447..240237	-	1791	WP_069239508.1	nitrite/sulfite reductase	-
WJ35_RS30255	240371..240517	-	147	Protein_264	transposase	-
WJ35_RS16170	241314..241541	+	228	WP_043292778.1	hypothetical protein	Antitoxin
WJ35_RS16175	241541..241972	+	432	WP_043292779.1	PIN domain-containing protein	Toxin
WJ35_RS16180	242387..242818	+	432	WP_011880199.1	hypothetical protein	-
WJ35_RS16185	242815..243348	+	534	WP_011880200.1	RES family NAD+ phosphorylase	-
WJ35_RS16190	243642..245339	-	1698	WP_069239509.1	site-specific integrase	-
WJ35_RS16195	245436..245840	-	405	WP_126221107.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	238043..307678	69635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-127)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15621.97 Da
• Isoelectric Point: 7.2342

>T58631 WP_043292779.1 NZ_CP013421:241541-241972 [Burkholderia ubonensis]
ITYLLDVNVLIALLDPAHVQHEPAHAWFGRVGHSAWATCPLTENGVLRIIGNPKYPNTMVSPASVAPLVVQLRSHAGHTF
WSDDVSLLDEKHVDATRLLSSAQLTDTYLLALARAHKGKLATFDRRLVVDAVPNGGKHLELIP

Nucleotide

• Length: 432 bp

>T58631 NZ_CP013421:241541-241972 [Burkholderia ubonensis]
ATTACCTACCTTCTCGACGTCAACGTACTCATTGCTTTGCTCGACCCGGCACACGTTCAACACGAGCCGGCGCATGCGTG
GTTTGGCCGGGTAGGCCATTCCGCATGGGCCACGTGCCCGCTCACCGAGAACGGCGTGCTTCGAATTATCGGCAATCCGA
AATACCCGAATACGATGGTCTCTCCTGCATCCGTTGCACCGCTTGTTGTGCAACTGCGGTCCCATGCTGGGCACACATTC
TGGTCGGACGATGTGAGCCTGCTCGACGAAAAACACGTCGACGCCACCCGTTTACTGTCCTCCGCTCAACTGACCGACAC
TTACCTCCTCGCGCTCGCTCGCGCACACAAAGGCAAGTTGGCGACCTTCGATCGCCGCTTGGTCGTCGACGCGGTTCCGA
ACGGCGGGAAGCATCTTGAACTGATTCCTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8080.14 Da
• Isoelectric Point: 8.9793

>AT58631 WP_043292778.1 NZ_CP013421:241314-241541 [Burkholderia ubonensis]
MRTTIDIDDDVLAFARERAKPGTSIGKVISDLARAALQRGTTNQSSRNGLPLMPVSATARPVTLDTVNQLRDDSL

Nucleotide

• Length: 228 bp

>AT58631 NZ_CP013421:241314-241541 [Burkholderia ubonensis]
ATGCGTACCACTATTGATATCGACGACGACGTCCTTGCCTTCGCGCGGGAGCGTGCAAAGCCCGGCACGAGTATCGGGAA
GGTCATTTCTGACCTCGCCCGTGCGGCCCTGCAGCGCGGAACAACGAACCAGTCGAGCCGAAACGGCTTGCCGTTGATGC
CGGTTTCCGCGACCGCCCGTCCGGTCACCCTTGATACCGTTAATCAACTTCGCGACGACTCGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B4LJE8