Detailed information of TA system TA058615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2452880..2453417
• Replicon: chromosome
• Accession: NZ_CP013413
• Organism: Burkholderia thailandensis strain 2002721643

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: AQ477_RS28820
• Protein ID: WP_004549731.1
• Coordinates: 2452880..2453149 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: AQ477_RS28825
• Protein ID: WP_045601930.1
• Coordinates: 2453133..2453417 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AQ477_RS28795	2448983..2449717	+	735	WP_009893914.1	hypothetical protein	-
AQ477_RS28815	2452387..2452830	+	444	WP_045601932.1	hypothetical protein	-
AQ477_RS28820	2452880..2453149	+	270	WP_004549731.1	BrnT family toxin	Toxin
AQ477_RS28825	2453133..2453417	+	285	WP_045601930.1	BrnA antitoxin family protein	Antitoxin
AQ477_RS28830	2453821..2454018	+	198	WP_045601929.1	hypothetical protein	-
AQ477_RS28835	2454108..2454368	+	261	WP_045598729.1	hypothetical protein	-
AQ477_RS28840	2454378..2456000	-	1623	WP_045598726.1	IS66 family transposase	-
AQ477_RS28845	2456100..2456453	-	354	WP_045598725.1	IS66 family insertion sequence element accessory protein TnpB	-
AQ477_RS28850	2456453..2456920	-	468	WP_045598724.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T58615 WP_004549731.1 NZ_CP013413:2452880-2453149 [Burkholderia thailandensis]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T58615 NZ_CP013413:2452880-2453149 [Burkholderia thailandensis]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCACGGGGTGTCGTTGGCGCTTGCGGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10655.06 Da
• Isoelectric Point: 5.8930

>AT58615 WP_045601930.1 NZ_CP013413:2453133-2453417 [Burkholderia thailandensis]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDVVDAFKATGEGWQTRM
NDALREWLKEHQPA

Nucleotide

• Length: 285 bp

>AT58615 NZ_CP013413:2453133-2453417 [Burkholderia thailandensis]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGTCGAGGTAAGCGCGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGATGTCGTCGACGCCTTCAAGGCGACGGGCGAAGGTTGGCAGACGCGGATG
AACGACGCGCTGCGCGAGTGGTTGAAGGAGCATCAACCGGCGTAG

Structures

Toxin

• AlphaFold DB: Q2T6G2