Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 2374311..2374966 Replicon chromosome
Accession NZ_CP013406
Organism Burkholderia lata strain FL-7-5-30-S1-D0

Toxin (Protein)


Gene name vapC Uniprot ID A0A6P3CB08
Locus tag WJ23_RS32455 Protein ID WP_069252092.1
Coordinates 2374311..2374730 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A228QU11
Locus tag WJ23_RS32460 Protein ID WP_069253001.1
Coordinates 2374727..2374966 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WJ23_RS32430 2369723..2370517 + 795 WP_069252089.1 AraC family transcriptional regulator -
WJ23_RS32435 2370704..2371681 + 978 WP_156814660.1 DMT family transporter -
WJ23_RS32440 2371890..2372438 + 549 WP_069252090.1 permease -
WJ23_RS32445 2372537..2372671 - 135 WP_006485835.1 entericidin A/B family lipoprotein -
WJ23_RS32450 2372905..2374197 + 1293 WP_069252091.1 aspartate carbamoyltransferase -
WJ23_RS32455 2374311..2374730 - 420 WP_069252092.1 type II toxin-antitoxin system VapC family toxin Toxin
WJ23_RS32460 2374727..2374966 - 240 WP_069253001.1 Arc family DNA-binding protein Antitoxin
WJ23_RS32465 2375286..2377529 + 2244 WP_069252093.1 TonB-dependent siderophore receptor -
WJ23_RS32470 2377533..2378312 + 780 WP_069252094.1 sel1 repeat family protein -
WJ23_RS32475 2378309..2378992 + 684 WP_069252095.1 Fe2+-dependent dioxygenase -
WJ23_RS32480 2379412..2379663 - 252 WP_069252096.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2354350..2383399 29049


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-122)

Antitoxin

Arc-like DNA binding domain

(9-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15167.50 Da        Isoelectric Point: 5.8883

>T58607 WP_069252092.1 NZ_CP013406:c2374730-2374311 [Burkholderia lata]
MILVDTNVMSEPLRREPSLAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLHQSIEQRVVPLFRGRILPFDD
AASKAYASIRARARASGSAIAPADVFIAATAEANGLIIATRDVAPFEAVGLRVINPWAS

Download         Length: 420 bp

>T58607 NZ_CP013406:c2374730-2374311 [Burkholderia lata]
ATGATCCTTGTTGATACGAACGTGATGTCCGAGCCGTTGCGGCGCGAGCCGAGCTTGGCCGTGATCGAATGGCTCGATGC
GCAGAACGTCGAAACGCTGTTTCTTGCTGCGATCAGCCTTGCAGAAATGCGCTTCGGTGTGGCGGCGTTACCGGAAGGGC
GCAGGCGCGACTGGCTTCACCAAAGCATCGAGCAGCGCGTCGTGCCGTTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCGGCCAGCAAGGCGTATGCGAGCATTCGAGCGCGTGCGCGTGCCTCAGGCAGTGCGATCGCGCCTGCCGATGTTTTTAT
CGCCGCGACGGCCGAGGCGAACGGACTCATCATTGCGACACGCGACGTCGCGCCATTCGAGGCGGTCGGCCTGCGGGTGA
TCAATCCGTGGGCGTCCTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8731.94 Da        Isoelectric Point: 5.6864

>AT58607 WP_069253001.1 NZ_CP013406:c2374966-2374727 [Burkholderia lata]
VPVITVRNLPDEVHRALRIRAAQHGRSTEAEVRDILEQAVLPGGRLKLGTLLAEIGREAGSVDFDVQRDKTLTDPMSFE

Download         Length: 240 bp

>AT58607 NZ_CP013406:c2374966-2374727 [Burkholderia lata]
GTGCCCGTGATTACCGTTCGAAATCTGCCTGATGAAGTGCACCGCGCGCTCCGAATTCGCGCGGCCCAGCATGGGCGCAG
CACCGAAGCAGAGGTGCGAGACATCCTCGAGCAGGCCGTTCTGCCGGGGGGGCGGCTCAAGCTGGGAACGCTGCTGGCGG
AGATCGGACGGGAGGCGGGCAGCGTCGATTTCGACGTTCAGCGCGACAAGACGCTAACCGACCCGATGAGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6P3CB08

Antitoxin

Source ID Structure
AlphaFold DB A0A228QU11

References