Detailed information of TA system TA058604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 846983..847624
• Replicon: chromosome
• Accession: NZ_CP013406
• Organism: Burkholderia lata strain FL-7-5-30-S1-D0

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: WJ23_RS25910
• Protein ID: WP_069251008.1
• Coordinates: 847286..847624 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A228GKS5
• Locus tag: WJ23_RS25905
• Protein ID: WP_069251007.1
• Coordinates: 846983..847282 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WJ23_RS25880	842146..843417	+	1272	WP_069252875.1	Rieske 2Fe-2S domain-containing protein	-
WJ23_RS25885	843432..843920	+	489	WP_069251004.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
WJ23_RS25890	843941..844267	+	327	WP_006479527.1	non-heme iron oxygenase ferredoxin subunit	-
WJ23_RS25895	844257..845477	+	1221	WP_069251005.1	FAD-dependent oxidoreductase	-
WJ23_RS25900	845648..846934	+	1287	WP_069251006.1	DUF445 domain-containing protein	-
WJ23_RS25905	846983..847282	-	300	WP_069251007.1	putative addiction module antidote protein	Antitoxin
WJ23_RS25910	847286..847624	-	339	WP_069251008.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WJ23_RS25915	847951..849591	+	1641	WP_069251009.1	acetolactate synthase large subunit	-
WJ23_RS25920	849621..851054	+	1434	WP_069251010.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (16-109)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12610.55 Da
• Isoelectric Point: 8.5041

>T58604 WP_069251008.1 NZ_CP013406:c847624-847286 [Burkholderia lata]
MVDILQSMPYCAPTFSIRTTDVFDAWFAGLPDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVILLCGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T58604 NZ_CP013406:c847624-847286 [Burkholderia lata]
ATGGTTGACATACTGCAATCCATGCCCTACTGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACGCCTGGTT
TGCCGGCCTGCCGGATCGCGTCGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTCTCGATGGGCAATCCGGGCGACT
GGAAGTCCGCCGGATCGCCGGTCGTCGAGATGCGGATCGACCACGGCCCAGGCTATCGCGTCTACTACGTCCGGCGCGGA
ACGATCTGGGTCATCCTGCTCTGCGGCGGCGATAAATCGACGCAACAGGCCGACATCCGCGCCGCACACGCGATGCTCGC
GCATCTCGACATGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10634.98 Da
• Isoelectric Point: 6.3217

>AT58604 WP_069251007.1 NZ_CP013406:c847282-846983 [Burkholderia lata]
MDKISTRPWDSADHLKTEDDMADYFEACLQEGGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGSKAHA

Nucleotide

• Length: 300 bp

>AT58604 NZ_CP013406:c847282-846983 [Burkholderia lata]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGATCATCTGAAAACCGAAGACGACATGGCCGACTACTTCGAAGC
GTGCCTGCAGGAAGGCGGCGACGATCCGGCCTTCATCGCACATGCGCTCGGCGTGATCGCACGGGCACGCGGCATGTCGC
AGGTCGCGCGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCCAGCTTCGGTACG
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCTCGAAAGCGCACGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A228GKS5