Detailed information of TA system TA058603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 614899..615707
• Replicon: chromosome
• Accession: NZ_CP013406
• Organism: Burkholderia lata strain FL-7-5-30-S1-D0

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: WJ23_RS24850
• Protein ID: WP_069252856.1
• Coordinates: 615201..615707 (+)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A228QM21
• Locus tag: WJ23_RS24845
• Protein ID: WP_069250837.1
• Coordinates: 614899..615204 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WJ23_RS24835	609979..611820	+	1842	WP_069250835.1	Hsp70 family protein	-
WJ23_RS24840	611826..614570	+	2745	WP_069250836.1	hsp70 family protein	-
WJ23_RS24845	614899..615204	+	306	WP_069250837.1	DUF1778 domain-containing protein	Antitoxin
WJ23_RS24850	615201..615707	+	507	WP_069252856.1	GNAT family N-acetyltransferase	Toxin
WJ23_RS24855	615933..616943	-	1011	WP_069250838.1	amidinotransferase	-
WJ23_RS24860	617003..618082	-	1080	WP_069250839.1	ornithine cyclodeaminase	-
WJ23_RS24865	618375..618806	+	432	WP_006479718.1	Lrp/AsnC family transcriptional regulator	-
WJ23_RS24870	618852..619445	-	594	WP_069250840.1	peroxidase-related enzyme	-
WJ23_RS24875	619442..619972	-	531	WP_069250841.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (47-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18147.89 Da
• Isoelectric Point: 9.0370

>T58603 WP_069252856.1 NZ_CP013406:615201-615707 [Burkholderia lata]
MTLRAPTPITLEHAQAGALDAFDSGEPSLDDWLKRRALRNHVSGASRTFVVCDDQRVVAYYALASGAVDVAAAPGRFRRN
MPDPVPVVVLARLAVDRAWQGRGLGRALLRDAGLRVMQAADTIGIRGLLAHALSADAQRFYLQVGFEPSPLDPMTVMITL
TDLRASLT

Nucleotide

• Length: 507 bp

>T58603 NZ_CP013406:615201-615707 [Burkholderia lata]
ATGACGCTGCGCGCGCCCACCCCGATTACGCTGGAACATGCGCAGGCGGGCGCCCTGGACGCCTTCGATTCGGGTGAGCC
GAGCCTGGACGATTGGCTCAAGCGGAGAGCCCTGCGTAACCATGTGAGCGGTGCGTCACGTACCTTCGTGGTGTGTGATG
ACCAGCGCGTCGTTGCCTACTACGCGCTAGCGTCTGGTGCGGTGGATGTCGCGGCCGCGCCCGGACGGTTTCGCCGCAAC
ATGCCGGACCCCGTACCGGTCGTTGTGTTGGCCAGGCTCGCGGTAGACCGGGCCTGGCAGGGCCGCGGACTGGGCCGCGC
GCTGCTGCGTGACGCCGGGCTACGGGTCATGCAGGCGGCCGACACCATCGGGATTCGCGGGCTGCTCGCACATGCACTGT
CGGCCGACGCGCAGCGCTTCTATCTGCAGGTCGGATTCGAGCCGTCGCCGCTTGACCCTATGACAGTCATGATCACACTG
ACCGATTTGCGTGCGTCGTTGACGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10821.12 Da
• Isoelectric Point: 7.0129

>AT58603 WP_069250837.1 NZ_CP013406:614899-615204 [Burkholderia lata]
MSATHSPPTKRDTLNLRVQPDERDLIDRAAAARGQNRTDFILSAARAAAEETLLEATLVRASPDAYAAFVARLEASPAPN
PQLRRTMQTPAPWDGSAGPVA

Nucleotide

• Length: 306 bp

>AT58603 NZ_CP013406:614899-615204 [Burkholderia lata]
ATGTCCGCTACCCATAGCCCCCCGACCAAGCGCGACACCCTGAACTTGCGCGTGCAGCCTGACGAACGCGACCTGATCGA
TCGCGCGGCCGCCGCACGCGGGCAGAACCGCACCGACTTCATCCTGTCCGCCGCTCGCGCGGCCGCTGAGGAAACCCTGC
TCGAGGCGACGCTGGTGCGCGCCAGTCCCGACGCGTATGCCGCGTTCGTCGCGCGGCTCGAGGCGTCACCGGCGCCGAAT
CCCCAGTTGCGGCGCACGATGCAGACGCCCGCGCCGTGGGACGGCTCGGCCGGCCCCGTCGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A228QM21