Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 879002..879534 Replicon chromosome
Accession NZ_CP013404
Organism Burkholderia lata strain FL-7-5-30-S1-D0

Toxin (Protein)


Gene name mazF Uniprot ID A0A228RF01
Locus tag WJ23_RS03945 Protein ID WP_069247237.1
Coordinates 879002..879325 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6P2ZAU8
Locus tag WJ23_RS03950 Protein ID WP_069247238.1
Coordinates 879322..879534 (-) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WJ23_RS03925 874909..875526 + 618 WP_069249224.1 phosphatase PAP2 family protein -
WJ23_RS03930 875523..876473 + 951 WP_069247234.1 fatty acid desaturase family protein -
WJ23_RS03935 876575..876883 + 309 WP_069247235.1 hypothetical protein -
WJ23_RS37140 877131..878165 - 1035 WP_083265841.1 relaxase/mobilization nuclease domain-containing protein -
WJ23_RS37145 878167..878691 - 525 WP_083265842.1 hypothetical protein -
WJ23_RS03945 879002..879325 - 324 WP_069247237.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
WJ23_RS03950 879322..879534 - 213 WP_069247238.1 antitoxin MazE family protein Antitoxin
WJ23_RS03955 879645..880124 - 480 WP_069249225.1 hypothetical protein -
WJ23_RS03960 880192..880587 - 396 WP_197523788.1 hypothetical protein -
WJ23_RS03965 880726..881046 + 321 WP_069247240.1 helix-turn-helix domain-containing protein -
WJ23_RS03975 881401..882150 - 750 WP_011351071.1 tol-pal system protein YbgF -
WJ23_RS03980 882170..882682 - 513 WP_011351072.1 peptidoglycan-associated lipoprotein Pal -
WJ23_RS03985 882700..883995 - 1296 WP_069247241.1 Tol-Pal system protein TolB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(7-67)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11636.71 Da        Isoelectric Point: 8.9366

>T58600 WP_069247237.1 NZ_CP013404:c879325-879002 [Burkholderia lata]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSSIVDAPLIRITVAPSEQNGLRMPTQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Download         Length: 324 bp

>T58600 NZ_CP013404:c879325-879002 [Burkholderia lata]
ATGAATCGGGGGAGCATTGTCACAGTTGCGCTCCAAGGTGACTTCGGCAAGGCCCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTCGCCGAACATCCCAGTGTAACGTTGCTGCTGATGTCGAGCTCAATAGTTGATGCGCCCTTGATACGCATCACTG
TTGCCCCATCCGAGCAGAACGGCTTGCGCATGCCAACCCAGATTGCCGTAGACAAAATGTTCACCGTGCGCCGCGAAAAA
ATCGGTCGGCCCATCGGCCACCTCGAAGATGAACTGATGGTTGCCGTCAATCGTTCGCTACTAGTTTTTCTAGGACTAGC
GTAA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 7970.91 Da        Isoelectric Point: 5.5492

>AT58600 WP_069247238.1 NZ_CP013404:c879534-879322 [Burkholderia lata]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRQPGFADECARQSRMVHQSIDESDLLDFIEQATDLGHSQ

Download         Length: 213 bp

>AT58600 NZ_CP013404:c879534-879322 [Burkholderia lata]
ATGGCAGCTACCACTTCCGAGCGTGTTCGAATCCACCGCGAGAATCTTCGTGCTGCCGGCCTGCGCCCAATTCAGATCTG
GGTGCCCGACGTCCGCCAACCGGGTTTCGCCGATGAATGCGCTCGGCAATCGCGCATGGTCCACCAGAGCATCGACGAGA
GTGATCTGCTCGATTTCATTGAACAGGCCACGGATCTCGGACACTCACAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A228RF01

Antitoxin

Source ID Structure
AlphaFold DB A0A6P2ZAU8

References