Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1074189..1074766 Replicon chromosome
Accession NZ_CP013403
Organism Burkholderia metallica strain FL-6-5-30-S1-D7

Toxin (Protein)


Gene name vapC Uniprot ID A0A1B4I2U1
Locus tag WJ16_RS24560 Protein ID WP_069259643.1
Coordinates 1074189..1074554 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1B4I2T5
Locus tag WJ16_RS24565 Protein ID WP_069259644.1
Coordinates 1074548..1074766 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WJ16_RS24540 1069752..1070651 + 900 WP_069259639.1 lipid A hydroxylase LpxO -
WJ16_RS24545 1070810..1071586 + 777 WP_069259640.1 extensin -
WJ16_RS24550 1071682..1073280 - 1599 WP_069259641.1 alkyl hydroperoxide reductase subunit F -
WJ16_RS24555 1073409..1073972 - 564 WP_069259642.1 peroxiredoxin -
WJ16_RS24560 1074189..1074554 - 366 WP_069259643.1 type II toxin-antitoxin system VapC family toxin Toxin
WJ16_RS24565 1074548..1074766 - 219 WP_069259644.1 CopG family transcriptional regulator Antitoxin
WJ16_RS24570 1074886..1075782 - 897 WP_069259645.1 LysR family transcriptional regulator -
WJ16_RS24575 1075960..1077129 + 1170 WP_069259646.1 branched-chain amino acid ABC transporter substrate-binding protein -
WJ16_RS24580 1077156..1077680 + 525 WP_069259647.1 L-2-amino-thiazoline-4-carboxylic acid hydrolase -
WJ16_RS24585 1077673..1078962 + 1290 WP_069259648.1 Zn-dependent hydrolase -
WJ16_RS24590 1079041..1079520 - 480 WP_069259649.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-104)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13551.64 Da        Isoelectric Point: 8.4485

>T58597 WP_069259643.1 NZ_CP013403:c1074554-1074189 [Burkholderia metallica]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISTISWMEVLVGTSARDEAPIRAWLSSFDVIALDSTIADRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNAKDFPPTEPGVRMPYRI

Download         Length: 366 bp

>T58597 NZ_CP013403:c1074554-1074189 [Burkholderia metallica]
ATGGTAAAAGCGCTCTTCGACACCAACATCCTGATCGACTATCTGGGCGGCGTGGAATCCGCCCGCGCGGAACTGGGCCG
CTACGACTATCGTGCGATCAGCACGATCTCGTGGATGGAGGTCCTGGTCGGTACGTCCGCACGCGACGAAGCCCCGATCC
GCGCGTGGCTTTCGTCGTTCGACGTCATCGCGCTCGACAGCACGATTGCCGATCGCGCCGTGACCATTCGAAAGGAACGC
CGCATCCGCCTGCCCGACGCGATCGTCTGGGCATCGGCGCAAGTAAACGGCCTGCTGCTCGTGTCGCGCAACGCGAAAGA
CTTTCCTCCCACCGAGCCCGGCGTCCGGATGCCGTACCGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8236.36 Da        Isoelectric Point: 4.5720

>AT58597 WP_069259644.1 NZ_CP013403:c1074766-1074548 [Burkholderia metallica]
MSRILIDLSNGQLDELAAIVETERRPRAAVIRDAIDAYIALHKRPLADDVFGLWKDRAVDGLAYQEELRSEW

Download         Length: 219 bp

>AT58597 NZ_CP013403:c1074766-1074548 [Burkholderia metallica]
ATGAGCCGGATTCTGATCGACCTGTCGAACGGCCAGCTCGACGAGCTGGCCGCCATCGTCGAGACTGAACGTCGTCCCCG
CGCCGCGGTCATCCGCGACGCGATCGATGCCTACATTGCCCTGCACAAGCGCCCGCTCGCCGACGACGTCTTCGGTCTCT
GGAAGGATCGCGCGGTCGACGGGCTCGCCTACCAGGAGGAATTGCGTTCGGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B4I2U1

Antitoxin

Source ID Structure
AlphaFold DB A0A1B4I2T5

References