Detailed information of TA system TA058592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2298894..2300114
• Replicon: chromosome
• Accession: NZ_CP013400
• Organism: Burkholderia seminalis strain FL-5-4-10-S1-D7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WJ12_RS30845
• Protein ID: WP_059556841.1
• Coordinates: 2299533..2300114 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: WJ12_RS30840
• Protein ID: WP_059556840.1
• Coordinates: 2298894..2299451 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WJ12_RS30815	2294366..2295097	-	732	WP_059556838.1	ribonuclease T2	-
WJ12_RS30820	2295328..2295717	+	390	WP_059556839.1	OsmC family protein	-
WJ12_RS30825	2295801..2296379	-	579	WP_034189318.1	hypothetical protein	-
WJ12_RS30830	2296474..2297877	-	1404	WP_034189317.1	membrane protein	-
WJ12_RS30835	2298218..2298856	+	639	WP_034189316.1	hypothetical protein	-
WJ12_RS30840	2298894..2299451	+	558	WP_059556840.1	helix-turn-helix domain-containing protein	Antitoxin
WJ12_RS30845	2299533..2300114	+	582	WP_059556841.1	HD domain-containing protein	Toxin
WJ12_RS30850	2300170..2300898	+	729	WP_069246050.1	class I SAM-dependent methyltransferase	-
WJ12_RS30855	2301161..2302345	+	1185	WP_049028329.1	macrolide transporter subunit MacA	-
WJ12_RS30860	2302348..2304420	+	2073	WP_069245978.1	MacB family efflux pump subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (6-147)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21417.05 Da
• Isoelectric Point: 5.0995

>T58592 WP_059556841.1 NZ_CP013400:2299533-2300114 [Burkholderia seminalis]
MNPDTIRARLDFLREAERLKDVLRSGHTSNGRPESTAEHSWRLCLMALVFADALPGIDMLKLLKLCIVHDLGEALHGDIP
AIEQAAHPDKSVHERDDLLTLAGSLDNAQRDAIVALWDEYEAAASPEARAAKALDKLETILQHNQGSNPPDFDYAFNLGY
GRRYTDAAPLFSAIREIVDADTQRRIDAGRQHA

Nucleotide

• Length: 582 bp

>T58592 NZ_CP013400:2299533-2300114 [Burkholderia seminalis]
ATGAACCCCGATACGATCCGCGCACGGCTCGATTTCCTGCGCGAAGCCGAACGCCTGAAGGACGTGCTGCGTAGCGGCCA
TACGTCGAACGGCCGCCCCGAAAGCACGGCCGAGCACAGCTGGCGGCTGTGCCTGATGGCGCTCGTGTTCGCCGATGCGC
TGCCCGGCATCGACATGCTGAAGCTGCTGAAGCTGTGCATCGTCCACGATCTCGGCGAGGCGCTGCACGGCGACATCCCC
GCGATCGAGCAGGCCGCGCATCCCGACAAGAGCGTGCACGAACGCGATGACCTGCTGACACTGGCCGGGTCGCTCGACAA
TGCGCAGCGCGACGCGATCGTCGCGCTGTGGGACGAATACGAAGCGGCCGCATCGCCGGAAGCGCGCGCGGCGAAGGCGC
TCGACAAGCTCGAGACGATCCTGCAGCACAACCAGGGCAGCAATCCGCCCGATTTCGACTACGCGTTCAATCTCGGCTAC
GGCCGGCGCTACACCGACGCCGCGCCGCTGTTCAGCGCGATCCGCGAGATCGTCGATGCCGACACGCAGCGCAGGATCGA
CGCGGGCAGGCAGCACGCGTAA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20203.13 Da
• Isoelectric Point: 10.2020

>AT58592 WP_059556840.1 NZ_CP013400:2298894-2299451 [Burkholderia seminalis]
MTASPLHTPPLGPQLKRWRALHRVKQSHAAELFGVAQSTISRWEAGRQQMSPDERATAERLLAARLDSAGDHALARLIAG
SAGRLHLVCDLTHRLLACSPARAAEFSQPLSMLLGTSLWRYATPEIVRMEAALDPLGWHERAGPPSVEFDTGANASRVVP
IRGSQCRWTRMTLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT58592 NZ_CP013400:2298894-2299451 [Burkholderia seminalis]
ATGACCGCCTCACCGCTTCACACGCCGCCGCTCGGCCCGCAACTCAAGCGCTGGCGCGCGCTGCACCGCGTCAAGCAAAG
CCATGCGGCCGAACTGTTCGGCGTCGCGCAATCGACGATCTCGCGCTGGGAAGCCGGCCGCCAGCAGATGTCGCCCGACG
AGCGCGCGACGGCCGAACGGCTGCTCGCCGCGCGTCTCGATTCCGCCGGCGACCACGCGCTCGCGCGGCTGATCGCGGGC
AGCGCGGGCCGCCTGCATCTCGTGTGCGACCTCACGCATCGGCTGCTCGCCTGTTCACCGGCGCGCGCGGCCGAGTTCTC
GCAGCCGCTTTCGATGCTGCTCGGCACGTCGCTGTGGCGCTATGCGACGCCGGAAATCGTCCGCATGGAGGCCGCGCTCG
ATCCGCTCGGCTGGCACGAGCGCGCGGGGCCGCCGAGCGTCGAGTTCGACACGGGCGCAAACGCATCGCGCGTCGTGCCG
ATCCGCGGCAGCCAGTGCCGGTGGACGCGCATGACGCTGTCGGACGGCTCGGCCGCGCGACTCGTCGAGACGCTTTAG