Detailed information of TA system TA058581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 828602..829243
• Replicon: chromosome
• Accession: NZ_CP013391
• Organism: Burkholderia contaminans strain FL-1-2-30-S1-D0

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: WI95_RS20970
• Protein ID: WP_122475171.1
• Coordinates: 828905..829243 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1J9PWC2
• Locus tag: WI95_RS20965
• Protein ID: WP_039344756.1
• Coordinates: 828602..828901 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WI95_RS20940	823766..825037	+	1272	WP_039344770.1	Rieske 2Fe-2S domain-containing protein	-
WI95_RS20945	825051..825539	+	489	WP_011355917.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
WI95_RS20950	825560..825886	+	327	WP_006479527.1	non-heme iron oxygenase ferredoxin subunit	-
WI95_RS20955	825876..827096	+	1221	WP_122475168.1	FAD-dependent oxidoreductase	-
WI95_RS20960	827267..828553	+	1287	WP_046549446.1	DUF445 domain-containing protein	-
WI95_RS20965	828602..828901	-	300	WP_039344756.1	putative addiction module antidote protein	Antitoxin
WI95_RS20970	828905..829243	-	339	WP_122475171.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WI95_RS20980	829575..831215	+	1641	WP_122475174.1	acetolactate synthase large subunit	-
WI95_RS20985	831245..832678	+	1434	WP_047851661.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (26-98)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12628.51 Da
• Isoelectric Point: 9.6882

>T58581 WP_122475171.1 NZ_CP013391:c829243-828905 [Burkholderia contaminans]
MVDILQSMPYSAPTFSIRTTDVFDTWFAGLQDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVHYVRRG
TIWVILLCGGDKSTQQANIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T58581 NZ_CP013391:c829243-828905 [Burkholderia contaminans]
ATGGTTGACATACTGCAATCCATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACACCTGGTT
TGCCGGCCTGCAGGATCGCGTCGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTCTCGATGGGCAATCCGGGCGACT
GGAAGTCCGCCGGCTCGCCGGTCGTCGAGATGCGGATCGACCACGGCCCGGGCTATCGCGTCCACTACGTCCGGCGCGGA
ACGATCTGGGTCATCCTGCTCTGCGGCGGCGATAAATCGACGCAACAGGCCAACATCCGCGCCGCGCACGCGATGCTCGC
GCATCTCGACATGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10649.01 Da
• Isoelectric Point: 6.3217

>AT58581 WP_039344756.1 NZ_CP013391:c828901-828602 [Burkholderia contaminans]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGSKAHA

Nucleotide

• Length: 300 bp

>AT58581 NZ_CP013391:c828901-828602 [Burkholderia contaminans]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGATCATCTGAAAACCGAAGACGACATGGCCGACTACTTCGAAGC
GTGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCACATGCGCTCGGCGTGATCGCGCGTGCACGCGGCATGTCGC
AGGTCGCGCGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCCAGCTTCGGCACG
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCTCGAAAGCGCACGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A1J9PWC2