Detailed information of TA system TA058572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1981173..1981710
• Replicon: chromosome
• Accession: NZ_CP013387
• Organism: Burkholderia sp. BDU6

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A1B4FN00
• Locus tag: WS70_RS27465
• Protein ID: WP_059472885.1
• Coordinates: 1981441..1981710 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A1B4FN04
• Locus tag: WS70_RS27460
• Protein ID: WP_059472886.1
• Coordinates: 1981173..1981457 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WS70_RS27430	1977627..1977821	+	195	Protein_1635	helix-turn-helix domain-containing protein	-
WS70_RS32220	1977840..1978226	-	387	WP_162499002.1	hypothetical protein	-
WS70_RS27435	1978179..1978670	+	492	WP_059472888.1	hypothetical protein	-
WS70_RS31980	1979450..1979737	-	288	WP_156438324.1	hypothetical protein	-
WS70_RS27450	1980481..1980939	+	459	WP_162499003.1	avidin/streptavidin family protein	-
WS70_RS27460	1981173..1981457	-	285	WP_059472886.1	BrnA antitoxin family protein	Antitoxin
WS70_RS27465	1981441..1981710	-	270	WP_059472885.1	BrnT family toxin	Toxin
WS70_RS27470	1981839..1982252	+	414	WP_059472884.1	hypothetical protein	-
WS70_RS27475	1982249..1982578	-	330	WP_059472883.1	hypothetical protein	-
WS70_RS27485	1983244..1983584	+	341	Protein_1644	site-specific integrase	-
WS70_RS27490	1983770..1984306	-	537	WP_082722294.1	SOS response-associated peptidase family protein	-
WS70_RS27495	1984509..1984916	+	408	WP_082722295.1	VOC family protein	-
WS70_RS27500	1985043..1985243	-	201	WP_059597496.1	hypothetical protein	-
WS70_RS32550	1985484..1985792	+	309	WP_203236008.1	transposase	-
WS70_RS32555	1985831..1985977	+	147	WP_156438111.1	hypothetical protein	-
WS70_RS31985	1986263..1986421	+	159	WP_159082984.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1981173..1985516	4343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10319.72 Da
• Isoelectric Point: 6.4600

>T58572 WP_059472885.1 NZ_CP013387:c1981710-1981441 [Burkholderia sp. BDU6]
MDIAFDPAKNETNVAKHGVSLAFAAQLDWADVLSYVDARRDYDEVREVGFGVIGDRFYCMMFKQRRDSMHIISIRKANKW
EAKSYVEQP

Nucleotide

• Length: 270 bp

>T58572 NZ_CP013387:c1981710-1981441 [Burkholderia sp. BDU6]
ATGGATATCGCTTTCGACCCGGCCAAGAACGAAACGAACGTTGCCAAGCATGGGGTTTCGTTGGCGTTTGCGGCGCAGCT
GGATTGGGCGGATGTGTTGTCCTACGTGGATGCCCGACGCGACTACGATGAAGTTCGCGAGGTCGGGTTCGGTGTGATCG
GCGATCGCTTTTACTGCATGATGTTCAAGCAGCGTCGCGACTCAATGCACATCATCAGCATTCGCAAGGCGAACAAGTGG
GAGGCCAAGAGCTATGTCGAGCAACCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10647.01 Da
• Isoelectric Point: 5.1847

>AT58572 WP_059472886.1 NZ_CP013387:c1981457-1981173 [Burkholderia sp. BDU6]
MSSNRKIVMPTDGEDAAINRGIAADSDTFEVPAEDFAKMARRDKRGRPPLEAPKMQLTVRYDIDIVDAFKATGEGWQTRM
NDALREWLKEHQPA

Nucleotide

• Length: 285 bp

>AT58572 NZ_CP013387:c1981457-1981173 [Burkholderia sp. BDU6]
ATGTCGAGCAACCGTAAGATCGTCATGCCGACGGACGGCGAAGATGCGGCAATCAACCGCGGCATCGCGGCCGACTCCGA
CACGTTCGAAGTGCCGGCAGAGGACTTTGCGAAGATGGCGCGTCGAGATAAGCGCGGCCGTCCGCCGCTTGAGGCTCCAA
AGATGCAATTGACCGTGCGCTACGACATCGACATCGTCGACGCCTTCAAGGCGACAGGCGAAGGTTGGCAGACGCGCATG
AACGACGCGCTGCGTGAATGGTTGAAGGAACATCAGCCGGCGTAG

Structures

Toxin

• AlphaFold DB: A0A1B4FN00

Antitoxin

• AlphaFold DB: A0A1B4FN04