Detailed information of TA system TA058570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-StbC
• Location: 1385367..1386003
• Replicon: chromosome
• Accession: NZ_CP013387
• Organism: Burkholderia sp. BDU6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1B4FLU5
• Locus tag: WS70_RS24825
• Protein ID: WP_059598345.1
• Coordinates: 1385367..1385783 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: WS70_RS24830
• Protein ID: WP_082722426.1
• Coordinates: 1385770..1386003 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WS70_RS24800	1380604..1382579	+	1976	Protein_1139	type VI secretion system tip protein VgrG	-
WS70_RS24810	1382752..1383387	-	636	Protein_1140	transposase	-
WS70_RS24820	1384325..1385070	-	746	Protein_1141	IS3 family transposase	-
WS70_RS31860	1385139..1385330	-	192	WP_059598346.1	hypothetical protein	-
WS70_RS24825	1385367..1385783	-	417	WP_059598345.1	type II toxin-antitoxin system VapC family toxin	Toxin
WS70_RS24830	1385770..1386003	-	234	WP_082722426.1	DNA-binding protein	Antitoxin
WS70_RS24835	1386536..1387059	+	524	Protein_1145	transposase	-
WS70_RS24840	1387171..1387953	-	783	WP_108034042.1	IS21-like element helper ATPase IstB	-
WS70_RS24845	1387963..1389486	-	1524	WP_082722130.1	IS21 family transposase	-
WS70_RS24850	1389736..1390020	-	285	Protein_1148	transposase	-
WS70_RS24860	1390302..1390748	+	447	WP_159082961.1	DUF4145 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1381488..1392022	10534
	-	inside	IScluster/Tn	-	-	1384316..1387953	3637

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15524.70 Da
• Isoelectric Point: 6.0901

>T58570 WP_059598345.1 NZ_CP013387:c1385783-1385367 [Burkholderia sp. BDU6]
MYLVDTNVISEIRKQGRANKGVLGFFHAATRDDIDLYLSVVTVGELRRGVELIRHRGDEPQATRLEKWLNTVLQEFGQNI
LPVDEEIGQVWGRLRVPQPEHALDKLIAATALIHDLTVVTRNVDDFAGTGARVLNPFQ

Nucleotide

• Length: 417 bp

>T58570 NZ_CP013387:c1385783-1385367 [Burkholderia sp. BDU6]
ATGTATTTGGTTGACACCAACGTCATCAGCGAGATTCGCAAACAAGGTCGGGCAAACAAGGGCGTGTTGGGTTTCTTCCA
TGCAGCGACGCGTGACGATATCGACCTGTACCTGTCCGTTGTGACGGTCGGTGAGTTGCGGCGAGGCGTCGAACTGATTC
GACATCGAGGGGATGAACCGCAGGCAACGCGCCTCGAAAAGTGGCTGAACACGGTGTTGCAAGAGTTCGGACAAAACATC
CTGCCGGTTGATGAAGAGATTGGTCAGGTGTGGGGGCGTCTGCGAGTACCGCAGCCGGAGCACGCACTGGACAAGTTGAT
TGCGGCAACCGCGTTGATCCATGACCTGACGGTCGTTACGCGCAACGTCGACGACTTTGCAGGAACAGGCGCTCGCGTCT
TGAACCCCTTCCAATAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8468.60 Da
• Isoelectric Point: 6.2206

>AT58570 WP_082722426.1 NZ_CP013387:c1386003-1385770 [Burkholderia sp. BDU6]
MANLVVRNLDDALVQSLKERAAAHGRSAEAEHREILAKALRRPQRKTFAEVLMAMPNVGDDADFVRVEDGEAGHVFG

Nucleotide

• Length: 234 bp

>AT58570 NZ_CP013387:c1386003-1385770 [Burkholderia sp. BDU6]
ATGGCAAATCTTGTGGTGAGGAATTTGGATGACGCGCTTGTCCAGAGCCTGAAAGAGCGCGCGGCGGCGCACGGCCGGAG
CGCGGAAGCCGAGCATCGCGAGATTCTTGCGAAGGCCCTGCGCCGGCCGCAACGCAAGACCTTCGCGGAAGTGCTGATGG
CGATGCCGAATGTGGGTGATGACGCCGATTTCGTGCGAGTGGAAGACGGCGAGGCAGGGCATGTATTTGGTTGA

Structures

Toxin

• AlphaFold DB: A0A1B4FLU5