Detailed information of TA system TA058568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 167939..168525
• Replicon: chromosome
• Accession: NZ_CP013385
• Organism: Burkholderia sp. LA-2-3-30-S1-D2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1B4F7M6
• Locus tag: WS66_RS29715
• Protein ID: WP_059602373.1
• Coordinates: 168142..168525 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: WS66_RS29710
• Protein ID: WP_082728941.1
• Coordinates: 167939..168145 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WS66_RS29695	163613..166021	-	2409	WP_082728940.1	HAD-IC family P-type ATPase	-
WS66_RS29700	166169..166621	-	453	WP_059602870.1	hypothetical protein	-
WS66_RS29705	166655..167437	-	783	WP_059602369.1	hypothetical protein	-
WS66_RS29710	167939..168145	+	207	WP_082728941.1	DUF2191 domain-containing protein	Antitoxin
WS66_RS29715	168142..168525	+	384	WP_059602373.1	PIN domain-containing protein	Toxin
WS66_RS29720	168731..169045	+	315	WP_059602377.1	hypothetical protein	-
WS66_RS29735	169751..170062	+	312	WP_059602380.1	hypothetical protein	-
WS66_RS29740	170113..170424	+	312	WP_059602383.1	hypothetical protein	-
WS66_RS29745	170672..172105	+	1434	WP_059602386.1	RNA polymerase factor sigma-54	-
WS66_RS29750	172176..172364	+	189	WP_059536983.1	DUF3008 family protein	-
WS66_RS29755	172419..172580	+	162	WP_027792525.1	DUF1328 domain-containing protein	-
WS66_RS29760	172577..172963	+	387	WP_059602389.1	DUF3022 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	160844..169045	8201

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14316.69 Da
• Isoelectric Point: 6.8528

>T58568 WP_059602373.1 NZ_CP013385:168142-168525 [Burkholderia sp. LA-2-3-30-S1-D2]
MSVLVDTSVWVEHFRRPLPALIQLLRVDDVLTHPLVMLELTCGTPPEPRARTLGDLALLRQTRLATPGEVADWIERERLY
GRGCGAVDCTLLASVLLTPSTKLWTRDRRLAQLAEQFGVHYLPPDLH

Nucleotide

• Length: 384 bp

>T58568 NZ_CP013385:168142-168525 [Burkholderia sp. LA-2-3-30-S1-D2]
ATGAGCGTTCTGGTCGACACCTCCGTCTGGGTCGAGCATTTCCGGCGACCGCTACCGGCGCTTATCCAACTGCTGCGCGT
CGATGATGTCTTGACGCATCCACTAGTGATGCTCGAGCTCACGTGCGGGACGCCGCCCGAACCGCGGGCCCGAACCTTGG
GAGATCTCGCGCTATTGCGCCAAACGCGCCTCGCGACGCCGGGCGAGGTAGCGGACTGGATCGAGCGGGAACGGCTGTAT
GGGCGCGGATGCGGTGCGGTGGATTGCACGCTGCTGGCGTCGGTGCTGTTGACCCCGAGCACTAAGCTCTGGACGCGCGA
TCGTCGGCTCGCGCAACTGGCGGAGCAATTCGGGGTTCACTACCTACCGCCCGACCTCCATTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7313.27 Da
• Isoelectric Point: 4.5932

>AT58568 WP_082728941.1 NZ_CP013385:167939-168145 [Burkholderia sp. LA-2-3-30-S1-D2]
MRTTVTVDDTLYARALELAEPGMSPADLFRAALETFVRVQAGQRLAALGGSAPDMPDVPRRNPDAADR

Nucleotide

• Length: 207 bp

>AT58568 NZ_CP013385:167939-168145 [Burkholderia sp. LA-2-3-30-S1-D2]
ATGCGTACCACCGTCACCGTCGACGACACTCTTTATGCGCGCGCACTCGAACTTGCCGAGCCGGGTATGTCGCCGGCCGA
CCTGTTTCGGGCTGCACTAGAAACGTTCGTGCGTGTCCAGGCCGGCCAACGCCTTGCCGCGCTTGGCGGTAGCGCACCCG
ACATGCCGGATGTGCCGCGTCGCAACCCAGATGCGGCTGACCGATGA

Structures

Toxin

• AlphaFold DB: A0A1B4F7M6