Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/-
Location 633135..634359 Replicon chromosome
Accession NZ_CP013383
Organism Burkholderia sp. LA-2-3-30-S1-D2

Toxin (Protein)


Gene name vapC Uniprot ID A0A1B4ETB6
Locus tag WS66_RS02890 Protein ID WP_059600171.1
Coordinates 633135..633716 (-) Length 194 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1B4EY29
Locus tag WS66_RS02895 Protein ID WP_059600331.1
Coordinates 633802..634359 (-) Length 186 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WS66_RS02865 628594..630123 + 1530 WP_059600175.1 CoA-acylating methylmalonate-semialdehyde dehydrogenase -
WS66_RS02870 630142..631044 + 903 WP_059600174.1 3-hydroxyisobutyrate dehydrogenase -
WS66_RS02875 631041..631832 + 792 WP_059600173.1 enoyl-CoA hydratase -
WS66_RS02880 631829..632968 + 1140 WP_059600172.1 enoyl-CoA hydratase/isomerase family protein -
WS66_RS02885 633040..633138 - 99 Protein_553 LysR family transcriptional regulator -
WS66_RS02890 633135..633716 - 582 WP_059600171.1 HD domain-containing protein Toxin
WS66_RS02895 633802..634359 - 558 WP_059600331.1 helix-turn-helix domain-containing protein Antitoxin
WS66_RS02900 634684..636087 + 1404 WP_059600330.1 hypothetical protein -
WS66_RS02905 636513..637244 + 732 WP_059600170.1 ribonuclease T2 -
WS66_RS02910 637312..637947 - 636 WP_059600169.1 cysteine hydrolase -
WS66_RS02915 638394..639308 - 915 WP_059600329.1 glutaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

5'-deoxynucleotidase YfbR/HDDC2

(8-153)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-61)


Sequences

Toxin        


Download         Length: 194 a.a.        Molecular weight: 21327.91 Da        Isoelectric Point: 4.8273

>T58565 WP_059600171.1 NZ_CP013383:c633716-633135 [Burkholderia sp. LA-2-3-30-S1-D2]
MNLDTIHARLDFLREAECLKDVLRSGHTSTGRAESTAEHSWRLCLMALVFADALPDVDTTKLLKLCVVHDLGEALHGDIP
AIEQAAHPDKNAHERDDLLTLTAGLDRVLRDEIVALWDEYEAAASPEARAAKALDKLETILQHNQGSNPPDFDYAFNLGY
GRRYTDAVPLFSTIRAIVDADTQRRVDARGNGA

Download         Length: 582 bp

>T58565 NZ_CP013383:c633716-633135 [Burkholderia sp. LA-2-3-30-S1-D2]
ATGAACCTCGACACGATCCACGCGCGGCTCGATTTCCTGCGCGAAGCCGAATGCCTGAAAGACGTGCTGCGCAGCGGCCA
TACGTCGACCGGGCGCGCCGAAAGCACGGCCGAACACAGCTGGCGGCTGTGCCTGATGGCGCTCGTGTTTGCCGATGCGC
TACCCGACGTCGACACGACGAAGCTGCTGAAGCTGTGCGTCGTCCACGATCTCGGCGAAGCGCTGCACGGCGACATCCCC
GCGATCGAGCAGGCCGCGCATCCGGACAAGAACGCACACGAACGCGACGACCTGCTGACGCTGACGGCCGGGCTCGATCG
CGTGCTGCGCGACGAGATCGTCGCACTGTGGGACGAATACGAGGCGGCGGCATCACCGGAAGCGCGCGCCGCGAAGGCAC
TCGACAAGCTCGAAACGATCCTCCAGCACAACCAGGGCAGCAATCCGCCGGATTTCGACTACGCGTTCAATCTCGGCTAC
GGCCGGCGCTACACCGACGCGGTGCCGCTATTCAGCACGATCCGTGCGATCGTCGACGCGGACACGCAACGCCGGGTCGA
CGCTCGCGGCAACGGCGCGTAA

Antitoxin


Download         Length: 186 a.a.        Molecular weight: 20338.41 Da        Isoelectric Point: 11.0874

>AT58565 WP_059600331.1 NZ_CP013383:c634359-633802 [Burkholderia sp. LA-2-3-30-S1-D2]
MRASPLHTPPLGPQLKRWRTLHRMKQSHAAELFGVAQSTISRWEAGIQQMSPDERAMAERLLAARLDSAGDHALARLIAS
STARMHLVCDLTHRLLACSPSRAAEFSRPLPTLLGTSLWRYATPEIVRMEAALDPLGWHDRAGPPSVEFATGANASRVVP
IRGSTCRWTRMTLSDGSAARLVETL

Download         Length: 558 bp

>AT58565 NZ_CP013383:c634359-633802 [Burkholderia sp. LA-2-3-30-S1-D2]
ATGCGCGCCTCACCGCTTCACACCCCGCCGCTCGGCCCGCAACTCAAGCGTTGGCGCACGCTGCATCGCATGAAGCAGAG
CCACGCGGCCGAGTTGTTCGGCGTCGCGCAATCGACGATTTCGCGCTGGGAAGCCGGCATCCAGCAGATGTCGCCCGACG
AGCGCGCGATGGCCGAGCGGCTGCTTGCCGCGCGCCTGGATTCAGCGGGCGACCACGCGCTCGCCCGGCTGATCGCGAGC
AGCACGGCCCGCATGCATCTCGTGTGCGACCTCACGCACCGCCTGCTCGCCTGCTCGCCATCGCGCGCGGCCGAGTTCTC
GCGGCCGCTGCCGACGTTGCTCGGCACATCGCTGTGGCGCTACGCGACGCCGGAAATCGTCCGCATGGAAGCCGCGCTCG
ACCCGCTTGGCTGGCACGATCGCGCGGGGCCGCCGAGCGTCGAATTCGCGACCGGCGCAAATGCATCGCGCGTCGTGCCG
ATCCGCGGCAGCACCTGCCGATGGACACGGATGACGCTGTCGGACGGCTCCGCCGCGCGGCTCGTCGAAACGTTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B4ETB6

Antitoxin

Source ID Structure
AlphaFold DB A0A1B4EY29

References