Detailed information of TA system TA058561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 157136..157785
• Replicon: plasmid pMSMB43
• Accession: NZ_CP013381
• Organism: Burkholderia sp. Bp5365 strain MSMB43

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AQ610_RS18995
• Protein ID: WP_006029599.1
• Coordinates: 157375..157785 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7U4P815
• Locus tag: AQ610_RS18990
• Protein ID: WP_006029598.1
• Coordinates: 157136..157378 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AQ610_RS18975	153495..154703	+	1209	WP_009916766.1	AAA family ATPase	-
AQ610_RS18980	154700..155668	+	969	WP_043283354.1	ParB/RepB/Spo0J family partition protein	-
AQ610_RS18985	155867..156628	-	762	WP_144411849.1	hypothetical protein	-
AQ610_RS18990	157136..157378	+	243	WP_006029598.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AQ610_RS18995	157375..157785	+	411	WP_006029599.1	type II toxin-antitoxin system VapC family toxin	Toxin
AQ610_RS36215	157863..158027	+	165	WP_156436729.1	hypothetical protein	-
AQ610_RS19005	158395..158853	-	459	WP_006029600.1	hypothetical protein	-
AQ610_RS19010	159151..159918	+	768	WP_006029601.1	hypothetical protein	-
AQ610_RS19015	159957..160685	+	729	WP_009916772.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	bspI2 / bspR2	1..305114	305114

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14767.04 Da
• Isoelectric Point: 5.1526

>T58561 WP_006029599.1 NZ_CP013381:157375-157785 [Burkholderia sp. Bp5365]
MTLYLLDTNILSNVIRDPRGACTARIGETPPEQVCTSIIVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIGANDMLIAAHALAVDAVLVTDNTAEFARVAGLPVENWLRPAT

Nucleotide

• Length: 411 bp

>T58561 NZ_CP013381:157375-157785 [Burkholderia sp. Bp5365]
ATGACGCTGTATCTGCTCGACACCAACATTTTGTCGAACGTGATCCGAGACCCGCGCGGCGCATGCACCGCCCGCATCGG
AGAAACACCGCCCGAACAAGTGTGCACCTCGATCATCGTGGCCGCGGAACTCCGGTTTGGCGTCTGGAAGCGCGGATCGT
CGACGCTCGCGCAGCGCGTCGAGCAACTGCTCGCGAGCTTGACCGTGTTGCCGCTGCAGCCTGACGCAGACCGTTGCTAC
GGCCGGTTGCGGGCCGAGCTCGAGAAGCAAGGCCAGTTGATCGGCGCCAACGACATGCTAATCGCCGCGCATGCGCTGGC
CGTGGACGCCGTCCTGGTCACCGACAACACGGCGGAATTTGCTCGGGTCGCGGGGCTGCCAGTCGAAAACTGGTTGCGCC
CGGCGACGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9309.54 Da
• Isoelectric Point: 4.8499

>AT58561 WP_006029598.1 NZ_CP013381:157136-157378 [Burkholderia sp. Bp5365]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWTPLDETFPDIDNLPAEPVDIE

Nucleotide

• Length: 243 bp

>AT58561 NZ_CP013381:157136-157378 [Burkholderia sp. Bp5365]
ATGCCAACCCATGACACCCGCCACGCGCGCCTGTTTCGCAATGGGCGCAGCCAGGCCGTGCGGATCCCGCGCGAGTTCGA
GCTACCGGTCTCGGAAGTCACTATCCACCGGGAAGGCCGGCGCTTGATTATCGAGCCCGTGGAGGCCGCGCCCCTGCGTG
AGCTGTTCGCCCAATGGACGCCGCTGGACGAAACCTTTCCCGACATCGATAACCTGCCGGCTGAACCGGTCGACATCGAA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4P815