Detailed information of TA system TA058546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 998481..999172
• Replicon: chromosome
• Accession: NZ_CP013377
• Organism: Burkholderia pseudomultivorans strain SUB-INT23-BP2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WS57_RS04210
• Protein ID: WP_009688932.1
• Coordinates: 998717..999172 (+)
• Length: 152 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A132F1C7
• Locus tag: WS57_RS04205
• Protein ID: WP_012216644.1
• Coordinates: 998481..998720 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WS57_RS04180	993705..994100	-	396	WP_059512574.1	type II toxin-antitoxin system VapC family toxin	-
WS57_RS04185	994097..994336	-	240	WP_040131292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
WS57_RS04190	994493..995176	-	684	WP_009688936.1	Fe2+-dependent dioxygenase	-
WS57_RS04195	995173..995928	-	756	WP_059512521.1	sel1 repeat family protein	-
WS57_RS04200	995932..998178	-	2247	WP_009688934.1	TonB-dependent siderophore receptor	-
WS57_RS04205	998481..998720	+	240	WP_012216644.1	Arc family DNA-binding protein	Antitoxin
WS57_RS04210	998717..999172	+	456	WP_009688932.1	type II toxin-antitoxin system VapC family toxin	Toxin
WS57_RS04215	999272..1000567	-	1296	WP_009688931.1	aspartate carbamoyltransferase	-
WS57_RS04220	1000802..1000936	+	135	WP_009688930.1	entericidin A/B family lipoprotein	-
WS57_RS04225	1001033..1001602	-	570	WP_040131298.1	hypothetical protein	-
WS57_RS04230	1001752..1002699	-	948	WP_059512523.1	DMT family transporter	-
WS57_RS04235	1002755..1003549	-	795	WP_059512524.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 152 a.a.
• Molecular weight: 16325.64 Da
• Isoelectric Point: 7.2062

>T58546 WP_009688932.1 NZ_CP013377:998717-999172 [Burkholderia pseudomultivorans]
MILVDTNVISEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLQQSIEHRVVPVFRGRILPFDD
AASQAYARIRAHARAGGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWASQGARVRQGIASK

Nucleotide

• Length: 456 bp

>T58546 NZ_CP013377:998717-999172 [Burkholderia pseudomultivorans]
ATGATCCTTGTTGATACGAACGTCATTTCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
GCAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGAC
GCCGGCGGGACTGGCTGCAGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGCATCCTGCCGTTCGACGAT
GCCGCGAGCCAGGCCTATGCGCGCATTCGCGCGCACGCCCGTGCCGGCGGCAACGCGATCGCGCCCGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGTGGGCGTCGCAGGGCGCGCGAGTGCGCCAGGGCATCGCGTCGAAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8716.94 Da
• Isoelectric Point: 5.1133

>AT58546 WP_012216644.1 NZ_CP013377:998481-998720 [Burkholderia pseudomultivorans]
MPVITVRNLPDEVHRALRVRAALHGRSTEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTPTDPMSFE

Nucleotide

• Length: 240 bp

>AT58546 NZ_CP013377:998481-998720 [Burkholderia pseudomultivorans]
ATGCCTGTGATTACCGTTCGAAATTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCGCTGCATGGGCGCAG
TACCGAGGCGGAAGTGCGCGACATCCTCGAGCAGGCCGTGCTGTCGGAAGGGCGCGTGAAGCTGGGAACCTTGCTGGCGG
AAATCGGGCGGGAGGCAGGCGGTGTCGATCTCGACATCCAGCGTGACAAGACGCCAACCGATCCGATGAGCTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A132F1C7