Detailed information of TA system TA058543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2759824..2761044
• Replicon: chromosome
• Accession: NZ_CP013376
• Organism: Burkholderia cepacia strain INT3-BP177

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U2FUP4
• Locus tag: WI67_RS28765
• Protein ID: WP_021162781.1
• Coordinates: 2760463..2761044 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: WI67_RS28760
• Protein ID: WP_069254842.1
• Coordinates: 2759824..2760381 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WI67_RS28735	2755220..2755951	-	732	WP_006498851.1	ribonuclease T2	-
WI67_RS28740	2756182..2756571	+	390	WP_069254840.1	OsmC family protein	-
WI67_RS28745	2756653..2757231	-	579	WP_021162777.1	hypothetical protein	-
WI67_RS28750	2757328..2758731	-	1404	WP_059484040.1	hypothetical protein	-
WI67_RS28755	2759092..2759769	+	678	WP_069254841.1	hypothetical protein	-
WI67_RS28760	2759824..2760381	+	558	WP_069254842.1	helix-turn-helix domain-containing protein	Antitoxin
WI67_RS28765	2760463..2761044	+	582	WP_021162781.1	HD domain-containing protein	Toxin
WI67_RS28770	2761100..2761828	+	729	WP_059485165.1	class I SAM-dependent methyltransferase	-
WI67_RS28775	2762116..2763309	+	1194	WP_069254843.1	macrolide transporter subunit MacA	-
WI67_RS28780	2763312..2765372	+	2061	WP_060280407.1	MacB family efflux pump subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-172)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21256.86 Da
• Isoelectric Point: 5.2812

>T58543 WP_021162781.1 NZ_CP013376:2760463-2761044 [Burkholderia cepacia]
MNPDTIHARLAFLREAERLKDVLRSGHTSAGRAESTAEHSWRLCLMALVFADALPGIDTLKLLKLCVVHDLGEALHGDIP
AIEQAAHPDKSAHERDDLLTLTAPLDPAQRDEIVALWDEYEAAASPEARAAKAFDKLETILQHTQGRNPPGFDYAFNLGY
GRRYTDAAPLFSAIRDIVDADTQRRIDASGQHA

Nucleotide

• Length: 582 bp

>T58543 NZ_CP013376:2760463-2761044 [Burkholderia cepacia]
ATGAACCCCGATACGATCCACGCGCGGCTCGCGTTCCTGCGCGAAGCCGAACGCCTGAAGGATGTGCTGCGCAGCGGCCA
CACGTCGGCCGGGCGCGCGGAAAGCACCGCCGAGCATAGCTGGCGGCTGTGCCTGATGGCACTCGTATTCGCCGATGCGC
TACCCGGCATCGACACGCTGAAGCTGCTGAAGCTGTGTGTCGTCCACGATCTCGGCGAGGCGCTGCACGGCGACATCCCC
GCGATCGAGCAGGCCGCGCACCCCGACAAGAGCGCGCACGAACGCGACGACCTGCTGACGCTGACCGCGCCGCTCGATCC
GGCGCAGCGCGACGAGATCGTCGCACTGTGGGATGAATACGAAGCGGCCGCGTCGCCGGAAGCGCGCGCGGCAAAAGCGT
TCGACAAGCTGGAGACGATCCTGCAGCACACCCAGGGCCGCAATCCGCCCGGTTTCGACTACGCGTTCAATCTCGGCTAC
GGCCGCCGCTATACCGACGCCGCACCGCTGTTCAGCGCGATCCGCGACATCGTCGACGCCGACACGCAACGCCGGATCGA
CGCGAGCGGGCAGCACGCGTAA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20052.02 Da
• Isoelectric Point: 11.1002

>AT58543 WP_069254842.1 NZ_CP013376:2759824-2760381 [Burkholderia cepacia]
MSASPLHAPPLGPQLKRWRALHRVKQSHAAELFGVAQSTISRWEAGLQQMSPGERATAERLLAARLDSAGDHALARLVAG
SAGRMHLVCDLTHRLLACSPARAAEFSQPLSMLLGTSLWRYATPAIVRMETALDTLGWHDRAGPPSVEFDTGANASRVVP
IRGSRCRWTRMTLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT58543 NZ_CP013376:2759824-2760381 [Burkholderia cepacia]
ATGTCTGCCTCACCGCTTCATGCACCGCCACTCGGCCCGCAACTCAAGCGCTGGCGTGCGCTGCACCGCGTGAAACAGAG
CCACGCGGCGGAACTGTTCGGCGTGGCGCAGTCGACGATCTCACGCTGGGAAGCCGGCCTGCAACAGATGTCGCCCGGCG
AACGCGCGACGGCCGAACGGCTGCTCGCCGCGCGCCTCGATTCCGCCGGCGATCACGCGCTTGCGCGGCTCGTCGCGGGC
AGCGCGGGCCGCATGCATCTCGTGTGCGACCTCACGCACCGGCTGCTCGCCTGTTCGCCTGCGCGTGCAGCCGAATTCTC
GCAACCGCTGTCGATGCTGCTCGGCACGTCGCTGTGGCGCTATGCGACGCCGGCAATCGTCCGCATGGAAACCGCGCTCG
ACACGCTCGGCTGGCATGATCGCGCGGGGCCGCCGAGCGTCGAATTCGACACCGGCGCGAATGCGTCGCGCGTCGTGCCG
ATCCGCGGCAGCCGGTGCCGGTGGACACGGATGACGTTGTCGGACGGGTCGGCCGCGCGGCTCGTGGAAACGCTGTAG

Structures

Toxin

• AlphaFold DB: U2FUP4