Detailed information of TA system TA058542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2546580..2547235
• Replicon: chromosome
• Accession: NZ_CP013376
• Organism: Burkholderia cepacia strain INT3-BP177

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U2HGC0
• Locus tag: WI67_RS33810
• Protein ID: WP_021158162.1
• Coordinates: 2546580..2546999 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2HMP5
• Locus tag: WI67_RS27915
• Protein ID: WP_021158161.1
• Coordinates: 2546996..2547235 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WI67_RS27885	2541864..2542658	+	795	WP_021158168.1	AraC family transcriptional regulator	-
WI67_RS27890	2543000..2543953	+	954	WP_069255196.1	DMT family transporter	-
WI67_RS27895	2544138..2544686	+	549	WP_021158166.1	hypothetical protein	-
WI67_RS27900	2544781..2544915	-	135	WP_021158165.1	entericidin A/B family lipoprotein	-
WI67_RS27905	2545149..2546444	+	1296	WP_021158163.1	aspartate carbamoyltransferase	-
WI67_RS33810	2546580..2546999	-	420	WP_021158162.1	type II toxin-antitoxin system VapC family toxin	Toxin
WI67_RS27915	2546996..2547235	-	240	WP_021158161.1	antitoxin VapB	Antitoxin
WI67_RS27920	2547546..2549792	+	2247	WP_059587041.1	TonB-dependent siderophore receptor	-
WI67_RS27925	2549796..2550563	+	768	WP_059587039.1	sel1 repeat family protein	-
WI67_RS27930	2550560..2551243	+	684	WP_069254805.1	Fe2+-dependent dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15212.57 Da
• Isoelectric Point: 5.9052

>T58542 WP_021158162.1 NZ_CP013376:c2546999-2546580 [Burkholderia cepacia]
MILVDTNVISEPLRREPNAAVIEWLDAQNVETLFLAAISLAEIRLGVAVLPEGRRREWLHQSIEQRVLPLFRGRILPFDD
AASKAYASLRARARAAGVAIAPSDGFIAGTAEANGLIVATRDVTPFEAMGIRVIDPWAR

Nucleotide

• Length: 420 bp

>T58542 NZ_CP013376:c2546999-2546580 [Burkholderia cepacia]
ATGATCCTTGTTGATACGAATGTCATTTCCGAACCGCTACGACGCGAGCCGAACGCGGCCGTGATCGAGTGGCTCGATGC
CCAGAATGTCGAAACCTTGTTTCTGGCTGCGATCAGTCTCGCGGAAATACGATTGGGCGTGGCTGTATTGCCGGAAGGAC
GCCGGCGAGAGTGGCTGCATCAGAGCATCGAGCAGCGCGTGCTGCCATTGTTTCGCGGACGAATCCTGCCATTCGACGAT
GCTGCGAGCAAAGCGTATGCGAGCCTTCGCGCACGCGCTCGTGCCGCAGGTGTCGCAATTGCACCATCCGATGGTTTTAT
CGCCGGCACGGCCGAAGCGAACGGCCTGATCGTCGCCACGCGCGATGTCACGCCGTTCGAGGCAATGGGAATTCGCGTAA
TCGACCCGTGGGCGCGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8629.79 Da
• Isoelectric Point: 5.6643

>AT58542 WP_021158161.1 NZ_CP013376:c2547235-2546996 [Burkholderia cepacia]
MPVITVRNLSDEVHRALRIRAAQHGRSTEAEVRDILEQAVLPGGRLKLGTMLAAIGQEAGGFDFDAQRDKTPADPMSFE

Nucleotide

• Length: 240 bp

>AT58542 NZ_CP013376:c2547235-2546996 [Burkholderia cepacia]
ATGCCGGTGATCACCGTCCGAAATCTGTCCGATGAAGTGCATCGAGCACTTCGCATTCGCGCGGCCCAACATGGGCGCAG
CACCGAAGCCGAGGTGCGCGACATTCTCGAACAGGCCGTTCTTCCGGGCGGGCGGCTCAAGCTGGGAACGATGCTGGCGG
CAATCGGGCAGGAGGCCGGCGGATTCGATTTCGACGCCCAGCGCGACAAGACGCCTGCCGATCCGATGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: U2HGC0

Antitoxin

• AlphaFold DB: U2HMP5