Detailed information of TA system TA058539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1317747..1318324
• Replicon: chromosome
• Accession: NZ_CP013376
• Organism: Burkholderia cepacia strain INT3-BP177

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WI67_RS22695
• Protein ID: WP_069254400.1
• Coordinates: 1317747..1318112 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2HEK9
• Locus tag: WI67_RS22700
• Protein ID: WP_021160898.1
• Coordinates: 1318106..1318324 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WI67_RS22675	1313402..1313956	+	555	WP_021160894.1	carboxymuconolactone decarboxylase family protein	-
WI67_RS22680	1314117..1314875	+	759	WP_069254399.1	extensin	-
WI67_RS22685	1315120..1316718	-	1599	WP_059492406.1	alkyl hydroperoxide reductase subunit F	-
WI67_RS22690	1316847..1317410	-	564	WP_006488685.1	peroxiredoxin	-
WI67_RS22695	1317747..1318112	-	366	WP_069254400.1	type II toxin-antitoxin system VapC family toxin	Toxin
WI67_RS22700	1318106..1318324	-	219	WP_021160898.1	hypothetical protein	Antitoxin
WI67_RS22705	1318415..1319311	-	897	WP_048026027.1	LysR family transcriptional regulator	-
WI67_RS22710	1319489..1320655	+	1167	WP_027791290.1	branched-chain amino acid ABC transporter substrate-binding protein	-
WI67_RS22715	1320715..1321239	+	525	WP_021160901.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
WI67_RS22720	1321232..1322497	+	1266	WP_069254401.1	Zn-dependent hydrolase	-
WI67_RS22725	1322585..1323067	-	483	WP_060322975.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13478.53 Da
• Isoelectric Point: 8.4389

>T58539 WP_069254400.1 NZ_CP013376:c1318112-1317747 [Burkholderia cepacia]
MVKALFDTNILIDYLSGVESARAELGRYDYRAISTISWMEVLVGTSAQDEAPIRAWLSSFDVIALDSPVANRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGVRVPYKL

Nucleotide

• Length: 366 bp

>T58539 NZ_CP013376:c1318112-1317747 [Burkholderia cepacia]
ATGGTAAAAGCTCTCTTCGATACCAACATCCTGATCGACTATCTGAGCGGCGTGGAATCCGCTCGTGCGGAACTGGGCCG
CTACGACTATCGGGCGATCAGCACGATCTCGTGGATGGAGGTCCTGGTCGGCACGTCCGCACAGGACGAGGCACCCATCC
GCGCGTGGCTGTCGTCGTTCGATGTCATCGCACTGGACAGCCCGGTTGCGAATCGTGCCGTGACAATCCGCAAGGAACGC
CGCATACGCCTTCCCGATGCAATCGTCTGGGCATCGGCACAAGTGAACGGTCTACTGCTCGTGTCGCGCAACACGAAGGA
CTTCCCTGCCACCGAACCCGGCGTTCGCGTGCCCTACAAGCTCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.51 Da
• Isoelectric Point: 5.0604

>AT58539 WP_021160898.1 NZ_CP013376:c1318324-1318106 [Burkholderia cepacia]
MSRILVDLSDGQLDELAVIVETQHRPRAAIIRDAIDAYIALHKHRLADDVFGLWKDRKVDGLTYQEELRSEW

Nucleotide

• Length: 219 bp

>AT58539 NZ_CP013376:c1318324-1318106 [Burkholderia cepacia]
ATGAGCCGAATTCTGGTCGATCTGTCGGACGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACATCGTCCCCG
CGCCGCAATCATCCGCGACGCGATCGATGCCTATATTGCCTTGCACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTCT
GGAAAGATCGCAAGGTCGACGGGCTCACCTATCAGGAAGAACTGCGCTCGGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: U2HEK9