Detailed information of TA system TA058538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 956668..957309
• Replicon: chromosome
• Accession: NZ_CP013376
• Organism: Burkholderia cepacia strain INT3-BP177

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: WI67_RS21120
• Protein ID: WP_042973286.1
• Coordinates: 956971..957309 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A2X1G6Z6
• Locus tag: WI67_RS21115
• Protein ID: WP_060309649.1
• Coordinates: 956668..956967 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WI67_RS21090	951782..953053	+	1272	WP_069254309.1	Rieske 2Fe-2S domain-containing protein	-
WI67_RS21095	953071..953556	+	486	WP_034196026.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
WI67_RS21100	953577..953903	+	327	WP_006479527.1	non-heme iron oxygenase ferredoxin subunit	-
WI67_RS21105	953893..955113	+	1221	WP_069254310.1	FAD-dependent oxidoreductase	-
WI67_RS21110	955325..956611	+	1287	WP_060343380.1	DUF445 domain-containing protein	-
WI67_RS21115	956668..956967	-	300	WP_060309649.1	putative addiction module antidote protein	Antitoxin
WI67_RS21120	956971..957309	-	339	WP_042973286.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WI67_RS21125	957649..959289	+	1641	WP_059591671.1	acetolactate synthase large subunit	-
WI67_RS21130	959318..960751	+	1434	WP_048022192.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (26-98)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12637.52 Da
• Isoelectric Point: 9.5686

>T58538 WP_042973286.1 NZ_CP013376:c957309-956971 [Burkholderia cepacia]
MVDIPQSMPYSAPTFSIRTTDVFDAWFAGLRDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVILLCGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T58538 NZ_CP013376:c957309-956971 [Burkholderia cepacia]
ATGGTTGACATACCGCAATCAATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACGCATGGTT
TGCCGGCCTGCGGGATCGCGTCGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTGTCGATGGGCAATCCGGGTGACT
GGAAATCCGCGGGCTCGCCGGTCGTCGAAATGCGGATCGACCACGGCCCCGGTTATCGCGTCTACTACGTCCGGCGCGGA
ACGATCTGGGTCATCCTGCTCTGCGGCGGCGATAAATCGACGCAACAGGCCGACATACGCGCCGCGCACGCGATGCTCGC
GCACCTCGACATGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10632.02 Da
• Isoelectric Point: 6.6378

>AT58538 WP_060309649.1 NZ_CP013376:c956967-956668 [Burkholderia cepacia]
MNKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT58538 NZ_CP013376:c956967-956668 [Burkholderia cepacia]
ATGAACAAGATCAGCACCCGGCCGTGGGATTCGGCCGACCATCTGAAGACCGAAGACGACATGGCCGACTACTTCGAAGC
GTGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCACGCGCACGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGTTGTACAAGGCGCTGTCGCACGACGGCAACCCCAGCTTCGGCACC
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCGCAAAAGCGCACGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1G6Z6