Detailed information of TA system TA058535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-VapB
• Location: 227521..228107
• Replicon: chromosome
• Accession: NZ_CP013374
• Organism: Burkholderia sp. NRF60-BP8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WS54_RS30720
• Protein ID: WP_082725006.1
• Coordinates: 227724..228107 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A807ES36
• Locus tag: WS54_RS30715
• Protein ID: WP_059780000.1
• Coordinates: 227521..227727 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WS54_RS30695	222862..223182	-	321	WP_034209167.1	hypothetical protein	-
WS54_RS30700	223656..224918	+	1263	WP_059779909.1	hypothetical protein	-
WS54_RS30705	225036..225878	-	843	WP_059779907.1	hypothetical protein	-
WS54_RS30710	226343..226942	+	600	WP_159086715.1	hypothetical protein	-
WS54_RS30715	227521..227727	+	207	WP_059780000.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
WS54_RS30720	227724..228107	+	384	WP_082725006.1	PIN domain-containing protein	Toxin
WS54_RS30725	228672..230717	+	2046	WP_059779903.1	transketolase	-
WS54_RS30730	230850..231164	+	315	WP_034209161.1	hypothetical protein	-
WS54_RS30735	231539..231886	-	348	WP_034209160.1	helix-turn-helix transcriptional regulator	-
WS54_RS30740	232014..232868	+	855	WP_059779902.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-115)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-43)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14350.58 Da
• Isoelectric Point: 6.8531

>T58535 WP_082725006.1 NZ_CP013374:227724-228107 [Burkholderia sp. NRF60-BP8]
MSVLVDTSVWVEHFRRSLPALIQLLSVDNVLTHPLVMLELTCGTPPEPRARTLGDLALLRQTRRATPGEVADWIERERLY
GRGCGAVDCTLLASVLLTPNTRLWTRDRRLAQLAEQFGVHYEPPDLH

Nucleotide

• Length: 384 bp

>T58535 NZ_CP013374:227724-228107 [Burkholderia sp. NRF60-BP8]
ATGAGTGTGTTGGTCGACACGTCCGTGTGGGTCGAGCATTTCCGGCGGTCACTACCAGCGCTTATCCAACTTCTGAGCGT
CGATAACGTCCTTACACATCCACTGGTGATGCTTGAGCTCACGTGCGGGACGCCGCCCGAACCGCGGGCACGAACGCTGG
GAGATCTCGCGTTATTGCGCCAAACGCGCCGCGCGACGCCAGGCGAAGTAGCGGACTGGATCGAGCGGGAACGGCTGTAT
GGGCGCGGATGCGGTGCCGTGGATTGCACGCTGCTTGCATCGGTGCTGTTGACCCCGAACACCCGACTCTGGACGCGCGA
TCGGCGGCTCGCGCAACTGGCAGAGCAATTCGGGGTTCACTACGAACCGCCTGACCTGCATTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7329.47 Da
• Isoelectric Point: 6.2573

>AT58535 WP_059780000.1 NZ_CP013374:227521-227727 [Burkholderia sp. NRF60-BP8]
MRTTVTVDDALYARALELAEPGIPPAELFRAALETFVRVQAGQRLAALGGRAPDMADVPRRAPEVIAR

Nucleotide

• Length: 207 bp

>AT58535 NZ_CP013374:227521-227727 [Burkholderia sp. NRF60-BP8]
ATGCGTACCACCGTCACCGTCGACGACGCTCTCTATGCGCGCGCACTCGAACTCGCCGAACCTGGCATTCCGCCGGCCGA
GCTATTCCGGGCGGCACTTGAAACCTTCGTGCGCGTCCAGGCCGGTCAACGCCTGGCCGCCCTGGGCGGTCGCGCGCCCG
ACATGGCAGATGTCCCGCGCCGCGCTCCTGAGGTCATTGCCCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A807ES36