Detailed information of TA system TA058527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1485749..1486388
• Replicon: chromosome
• Accession: NZ_CP013372
• Organism: Burkholderia sp. NRF60-BP8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WS54_RS06880
• Protein ID: WP_179949311.1
• Coordinates: 1485749..1486165 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A807EDK5
• Locus tag: WS54_RS06885
• Protein ID: WP_034204757.1
• Coordinates: 1486152..1486388 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WS54_RS06845	1482016..1482915	-	900	WP_059783413.1	AraC family transcriptional regulator	-
WS54_RS06850	1483082..1483861	+	780	WP_059783411.1	SDR family oxidoreductase	-
WS54_RS06865	1484366..1484629	-	264	WP_059783409.1	translation initiation factor IF-1	-
WS54_RS06875	1485098..1485301	-	204	WP_006483056.1	cold-shock protein	-
WS54_RS06880	1485749..1486165	-	417	WP_179949311.1	type II toxin-antitoxin system VapC family toxin	Toxin
WS54_RS06885	1486152..1486388	-	237	WP_034204757.1	DNA-binding protein	Antitoxin
WS54_RS06890	1486874..1488100	+	1227	WP_059783405.1	acyltransferase	-
WS54_RS06895	1488112..1488789	-	678	WP_082725170.1	hypothetical protein	-
WS54_RS06910	1489616..1491001	+	1386	WP_059783401.1	DHA2 family efflux MFS transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15414.69 Da
• Isoelectric Point: 6.8816

>T58527 WP_179949311.1 NZ_CP013372:c1486165-1485749 [Burkholderia sp. NRF60-BP8]
VFLIDTNVISEIRKGKRTNRGVRAFFRQAEADASPLYLSVVTVAELRRGVDLIRHRGDHSQASALEAWIAMILSGYAPNI
LPVDIEISQMWGHLRVPDPTHELDKLIAATALINDLTVVTRNVDDFARTGVRLLNPFD

Nucleotide

• Length: 417 bp

>T58527 NZ_CP013372:c1486165-1485749 [Burkholderia sp. NRF60-BP8]
GTGTTTTTGATTGATACGAACGTCATCAGCGAAATCAGGAAAGGCAAGCGCACCAACCGCGGCGTGCGGGCATTCTTCAG
GCAGGCCGAAGCCGACGCGAGCCCGCTTTACCTGTCGGTCGTGACGGTGGCCGAGTTGCGGCGCGGCGTCGATCTGATCC
GTCATCGCGGCGATCACAGCCAGGCGTCGGCGCTCGAGGCCTGGATCGCGATGATCCTGTCCGGCTATGCGCCGAACATC
CTGCCGGTCGATATCGAGATCAGCCAGATGTGGGGGCACTTGCGCGTGCCCGATCCGACGCACGAACTCGACAAGCTGAT
CGCAGCCACCGCGCTGATCAACGATCTGACGGTCGTCACGCGCAACGTCGACGATTTCGCCCGCACCGGCGTCCGGTTGC
TGAATCCGTTCGACTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8696.76 Da
• Isoelectric Point: 5.0774

>AT58527 WP_034204757.1 NZ_CP013372:c1486388-1486152 [Burkholderia sp. NRF60-BP8]
MANLLVRNVDDTIVQSLREQAAANGRSAEAEHRAILADALGRPKRRTFAQVLMSMPEVGEDADFQRVQDSGEVRRVFD

Nucleotide

• Length: 237 bp

>AT58527 NZ_CP013372:c1486388-1486152 [Burkholderia sp. NRF60-BP8]
ATGGCAAATCTTCTGGTGCGTAACGTGGATGACACTATCGTTCAAAGCCTGCGCGAGCAGGCCGCGGCCAATGGCAGGAG
CGCCGAGGCCGAACATCGAGCCATTCTGGCCGACGCGCTCGGCCGGCCGAAGCGGCGGACTTTCGCGCAAGTGCTGATGA
GCATGCCGGAGGTCGGCGAAGACGCGGATTTTCAACGTGTTCAGGATTCAGGCGAGGTCCGGCGTGTTTTTGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A807EDK5