Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1033398..1034044 Replicon chromosome
Accession NZ_CP013372
Organism Burkholderia sp. NRF60-BP8

Toxin (Protein)


Gene name vapC Uniprot ID A0A807ECK2
Locus tag WS54_RS04810 Protein ID WP_059783877.1
Coordinates 1033637..1034044 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A807ECJ5
Locus tag WS54_RS04805 Protein ID WP_027782744.1
Coordinates 1033398..1033640 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WS54_RS04790 1029378..1030784 + 1407 WP_034205094.1 aspartate ammonia-lyase -
WS54_RS04795 1030888..1032393 + 1506 WP_059783881.1 amino acid permease -
WS54_RS04800 1032451..1033140 - 690 WP_059783879.1 phospholipase -
WS54_RS04805 1033398..1033640 + 243 WP_027782744.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
WS54_RS04810 1033637..1034044 + 408 WP_059783877.1 type II toxin-antitoxin system VapC family toxin Toxin
WS54_RS04815 1034161..1034388 - 228 WP_082725155.1 hypothetical protein -
WS54_RS04820 1034446..1036833 + 2388 WP_059783876.1 MCP four helix bundle domain-containing protein -
WS54_RS04825 1036856..1037584 + 729 WP_059785064.1 chemotaxis protein -
WS54_RS04830 1037581..1038159 + 579 WP_059783873.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-121)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14803.99 Da        Isoelectric Point: 6.7598

>T58525 WP_059783877.1 NZ_CP013372:1033637-1034044 [Burkholderia sp. NRF60-BP8]
MKFLLDTNAVIAILKGEPAMLARLHAWQPADFGIPAIVAHELYYGAYKSRRAAANVARVDALQFEVVPFDTEDAQHAGEI
RAHLIAAGAPIGPYDALIAGQVRARHLVLVTHNVRGFGRVPRLQFEDWLAEPNEG

Download         Length: 408 bp

>T58525 NZ_CP013372:1033637-1034044 [Burkholderia sp. NRF60-BP8]
ATGAAGTTCCTGCTGGACACCAACGCGGTGATCGCCATTCTCAAGGGCGAGCCGGCGATGCTGGCCCGTCTGCATGCGTG
GCAGCCGGCCGATTTCGGGATTCCTGCGATCGTCGCGCATGAGCTTTATTACGGCGCGTACAAGAGCCGGCGAGCGGCAG
CGAACGTCGCGCGTGTCGACGCGCTTCAGTTCGAGGTCGTACCGTTCGACACCGAGGACGCGCAGCATGCGGGCGAAATT
CGTGCACATCTGATCGCGGCCGGCGCGCCGATCGGCCCTTACGACGCGTTGATCGCAGGGCAGGTGCGCGCCCGGCATCT
CGTGCTCGTCACGCACAACGTCCGCGGATTCGGGCGGGTTCCGCGGTTGCAATTCGAGGACTGGCTCGCCGAACCGAACG
AAGGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8862.98 Da        Isoelectric Point: 5.0197

>AT58525 WP_027782744.1 NZ_CP013372:1033398-1033640 [Burkholderia sp. NRF60-BP8]
MDTAKIFKHGGSQAVRLPKDFRFDTAEVRIRRHGASVILEPVPQDWAWLTPLIGPVDADFEAAATTQPSAQERSGLDVFE

Download         Length: 243 bp

>AT58525 NZ_CP013372:1033398-1033640 [Burkholderia sp. NRF60-BP8]
ATGGATACGGCAAAGATCTTCAAGCATGGCGGCTCGCAGGCCGTCCGGCTGCCGAAGGATTTCCGCTTCGATACGGCCGA
GGTGCGCATCCGCCGCCATGGGGCGTCCGTCATTCTTGAACCGGTGCCGCAAGACTGGGCATGGCTGACACCGCTGATCG
GCCCGGTCGACGCCGATTTCGAGGCTGCCGCGACCACGCAGCCGTCCGCTCAGGAGCGTTCGGGCCTGGATGTGTTCGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A807ECK2

Antitoxin

Source ID Structure
AlphaFold DB A0A807ECJ5

References