Detailed information of TA system TA058524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 727526..728146
• Replicon: chromosome
• Accession: NZ_CP013372
• Organism: Burkholderia sp. NRF60-BP8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: WS54_RS03370
• Protein ID: WP_108041857.1
• Coordinates: 727829..728146 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A807EDK8
• Locus tag: WS54_RS03365
• Protein ID: WP_059784215.1
• Coordinates: 727526..727825 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WS54_RS03335	723069..723545	+	477	WP_034205320.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
WS54_RS03340	723581..723907	+	327	WP_059784224.1	non-heme iron oxygenase ferredoxin subunit	-
WS54_RS03345	723897..725117	+	1221	WP_034205319.1	FAD-dependent oxidoreductase	-
WS54_RS03350	725226..726512	+	1287	WP_059784221.1	DUF445 domain-containing protein	-
WS54_RS03355	726546..726848	-	303	WP_059784218.1	HigA family addiction module antidote protein	-
WS54_RS03360	726861..727139	-	279	WP_034205316.1	type II toxin-antitoxin system RelE/ParE family toxin	-
WS54_RS03365	727526..727825	-	300	WP_059784215.1	putative addiction module antidote protein	Antitoxin
WS54_RS03370	727829..728146	-	318	WP_108041857.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WS54_RS03380	728542..730182	+	1641	WP_034205314.1	acetolactate synthase large subunit	-
WS54_RS03385	730212..731645	+	1434	WP_059784211.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (9-100)

Antitoxin

• Uncharacterized protein HI1420 (5-93)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11773.53 Da
• Isoelectric Point: 9.6879

>T58524 WP_108041857.1 NZ_CP013372:c728146-727829 [Burkholderia sp. NRF60-BP8]
MPYSSPAFSIRTTDVFDTWFAGLPDRIAKRRIQARIDRLSMGNPGDWKSAGAPIVEMRIDHGPGYRVYSVRRATIWVILL
CGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 318 bp

>T58524 NZ_CP013372:c728146-727829 [Burkholderia sp. NRF60-BP8]
ATGCCTTACAGTTCCCCGGCATTCAGCATCCGGACCACCGACGTCTTCGACACCTGGTTTGCCGGCCTGCCCGATCGCAT
CGCCAAGCGCCGCATCCAGGCGCGCATCGACCGCCTGTCGATGGGCAATCCCGGCGACTGGAAATCCGCCGGCGCGCCCA
TCGTGGAAATGCGCATCGACCATGGGCCCGGCTACCGCGTCTACTCCGTCAGGCGCGCGACGATATGGGTCATCCTGCTT
TGCGGCGGCGATAAATCGACGCAGCAGGCCGACATACGCGCCGCGCATGCAATGCTCGCGCACCTCGACATGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10607.06 Da
• Isoelectric Point: 6.8692

>AT58524 WP_059784215.1 NZ_CP013372:c727825-727526 [Burkholderia sp. NRF60-BP8]
MNKIKTRPWDSAEHLKTEDDMADYFDACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAAA

Nucleotide

• Length: 300 bp

>AT58524 NZ_CP013372:c727825-727526 [Burkholderia sp. NRF60-BP8]
ATGAACAAGATCAAGACGCGGCCATGGGATTCGGCCGAACACCTGAAAACCGAAGACGACATGGCCGACTACTTCGACGC
ATGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCCCGCGCACGCGGCATGTCGC
AGGTCGCGCGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTCATCAAGGCGCTCGGCCTGCAATTGCACGGCGCCAAGGCTGCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A807EDK8