Detailed information of TA system TA058522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 151159..151817
• Replicon: chromosome
• Accession: NZ_CP013372
• Organism: Burkholderia sp. NRF60-BP8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WS54_RS00720
• Protein ID: WP_059503853.1
• Coordinates: 151159..151590 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: WS54_RS00725
• Protein ID: WP_027781942.1
• Coordinates: 151590..151817 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WS54_RS00705	146728..148125	-	1398	WP_059786486.1	ATP-dependent RNA helicase DbpA	-
WS54_RS00710	148122..148319	-	198	WP_006483193.1	hypothetical protein	-
WS54_RS00715	148768..150432	-	1665	WP_179955198.1	malate dehydrogenase (quinone)	-
WS54_RS00720	151159..151590	-	432	WP_059503853.1	PIN domain-containing protein	Toxin
WS54_RS00725	151590..151817	-	228	WP_027781942.1	DUF2191 domain-containing protein	Antitoxin
WS54_RS00730	152362..152784	-	423	WP_059503706.1	NUDIX domain-containing protein	-
WS54_RS00735	153074..153943	-	870	WP_059503708.1	mechanosensitive ion channel family protein	-
WS54_RS00740	154247..155620	+	1374	WP_059786493.1	M23 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	331..371410	371079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-127)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15625.83 Da
• Isoelectric Point: 6.9770

>T58522 WP_059503853.1 NZ_CP013372:c151590-151159 [Burkholderia sp. NRF60-BP8]
ITYLLDVNVLIALLDPSHVQHESAHAWFGRVGYASWATCPLTENGVLRIIGHPKYPNTMGSPVSVAPLVAQLRGHAGHTF
WPDDVSLLDAEHVDATRLLSSAQLTDTYLLALARAHSGKLASFDRRLVVDAVPNGRKHFELIS

Nucleotide

• Length: 432 bp

>T58522 NZ_CP013372:c151590-151159 [Burkholderia sp. NRF60-BP8]
ATCACCTACCTTCTCGACGTCAATGTTCTTATTGCATTGCTGGACCCTTCGCACGTTCAGCACGAATCCGCGCACGCATG
GTTCGGTCGCGTCGGATACGCTAGCTGGGCCACATGCCCCCTGACCGAGAACGGCGTTCTTCGGATCATCGGTCATCCAA
AATATCCGAATACGATGGGTTCGCCGGTATCCGTTGCGCCGCTCGTCGCGCAACTGCGGGGCCACGCAGGCCATACTTTC
TGGCCGGACGATGTGAGCTTGCTCGACGCAGAACACGTCGATGCGACTCGCTTGTTGTCCTCTGCGCAGCTGACCGACAC
GTATCTGCTCGCGCTCGCTCGCGCGCACAGCGGCAAGCTCGCGAGCTTCGATCGGCGACTTGTCGTCGATGCAGTGCCGA
ACGGCAGAAAACACTTCGAATTGATTTCTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8129.18 Da
• Isoelectric Point: 7.0095

>AT58522 WP_027781942.1 NZ_CP013372:c151817-151590 [Burkholderia sp. NRF60-BP8]
MRTTIDIDDDVLAFARERARPGTSIGKVISELARAALQRTPANQTSRNGLPLMPVSSSAHPVTLDTVNQLRDDSL

Nucleotide

• Length: 228 bp

>AT58522 NZ_CP013372:c151817-151590 [Burkholderia sp. NRF60-BP8]
ATGCGTACCACGATCGATATCGATGACGACGTCCTCGCATTCGCCCGGGAACGTGCAAGGCCGGGTACAAGTATCGGCAA
GGTCATTTCCGAACTCGCTCGCGCCGCATTGCAGCGAACGCCGGCCAACCAGACGAGCCGCAACGGGTTACCTCTGATGC
CGGTCTCGTCGTCAGCCCACCCGGTCACGCTTGATACTGTTAATCAACTTCGTGACGATTCGCTTTGA