Detailed information of TA system TA058520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2307948..2309175
• Replicon: chromosome
• Accession: NZ_CP013371
• Organism: Burkholderia ubonensis strain RF23-BP41

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WI31_RS31485
• Protein ID: WP_059656801.1
• Coordinates: 2308594..2309175 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: WI31_RS31480
• Protein ID: WP_059656501.1
• Coordinates: 2307948..2308505 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WI31_RS31455	2303152..2304090	-	939	WP_059611370.1	S1C family serine protease	-
WI31_RS31460	2304388..2305281	+	894	WP_059656495.1	LysR family transcriptional regulator	-
WI31_RS31465	2305411..2306043	+	633	WP_059656799.1	LysE family translocator	-
WI31_RS31470	2306223..2306639	-	417	WP_059656497.1	hypothetical protein	-
WI31_RS31475	2306971..2307702	-	732	WP_059656499.1	ribonuclease T2	-
WI31_RS31480	2307948..2308505	+	558	WP_059656501.1	helix-turn-helix domain-containing protein	Antitoxin
WI31_RS31485	2308594..2309175	+	582	WP_059656801.1	HD domain-containing protein	Toxin
WI31_RS31490	2309428..2310612	+	1185	WP_059656503.1	macrolide transporter subunit MacA	-
WI31_RS31495	2310615..2312672	+	2058	WP_059656505.1	MacB family efflux pump subunit	-
WI31_RS31500	2312697..2314067	+	1371	WP_088503099.1	efflux transporter outer membrane subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-171)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-61)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21276.00 Da
• Isoelectric Point: 5.5340

>T58520 WP_059656801.1 NZ_CP013371:2308594-2309175 [Burkholderia ubonensis]
MTPDTIHARLAFLREAERLKDVLRSGHTSTGRAESTAEHSWRLCLMALVFADALDGVDPLKLLKLCVVHDLGEALHGDIP
AVEQAAHPDKSAHERRDLLALTAGLDAVLRDEIVALWDEYEQAVSPEAKAVKALDKLETILQHNQGDNPPDFDYAFNLGY
GRRHTDASPLFRAIRAIVDADTQSRIDAGARRA

Nucleotide

• Length: 582 bp

>T58520 NZ_CP013371:2308594-2309175 [Burkholderia ubonensis]
ATGACTCCCGACACGATCCACGCGCGACTCGCGTTCCTGCGCGAAGCGGAACGCCTGAAGGACGTGCTGCGCAGCGGCCA
CACGTCGACCGGCCGCGCGGAAAGCACGGCCGAGCACAGCTGGCGGCTGTGCCTGATGGCGCTGGTGTTCGCCGACGCGC
TCGACGGCGTCGACCCGCTGAAGCTGCTGAAGCTGTGCGTCGTCCACGACCTCGGCGAGGCGCTGCACGGCGACATCCCG
GCGGTCGAGCAGGCCGCGCATCCGGACAAGAGCGCGCACGAGCGGCGCGACCTGCTCGCGCTGACCGCCGGCCTCGACGC
CGTGCTGCGCGACGAGATCGTCGCGCTGTGGGACGAATACGAGCAGGCCGTGTCGCCGGAAGCGAAGGCCGTCAAGGCGC
TCGACAAGCTGGAAACGATCCTGCAGCACAACCAGGGCGACAATCCGCCGGACTTCGACTACGCGTTCAATCTCGGCTAC
GGCCGGCGCCATACGGACGCGAGCCCGCTGTTCCGCGCGATCCGGGCGATCGTCGACGCCGACACGCAAAGCCGGATCGA
CGCCGGCGCACGGCGCGCATGA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20196.20 Da
• Isoelectric Point: 9.4428

>AT58520 WP_059656501.1 NZ_CP013371:2307948-2308505 [Burkholderia ubonensis]
MTDSPLQTPPLGPLLKRWRLLRRVKQSHAAELFGVAQSTISRWEAGVQQMSPDERAIAERLLAARLDSAGDRALARLVGD
HPGRAHLVCDLTHRLLACSSLRAAEFAKPLPTMLGASLWRYATPEIAALEASLDTLGWRDSAGPPSVEFDSGANASRIVP
IHRSTCRWTRMILSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT58520 NZ_CP013371:2307948-2308505 [Burkholderia ubonensis]
ATGACCGACTCGCCACTTCAAACGCCGCCGCTCGGCCCGCTGCTGAAGCGCTGGCGGCTGCTGCGCCGCGTGAAGCAGAG
CCACGCGGCCGAGCTGTTCGGCGTCGCGCAGTCGACGATTTCCCGCTGGGAAGCCGGCGTCCAGCAGATGTCGCCCGACG
AGCGCGCGATCGCCGAGCGCCTGCTCGCGGCGCGGCTCGATTCCGCCGGCGACCGCGCGCTCGCGCGGCTCGTCGGCGAC
CATCCCGGCCGCGCGCACCTCGTGTGCGACCTCACGCATCGGCTGCTGGCGTGCTCGTCGTTGCGCGCCGCCGAGTTCGC
GAAACCGCTGCCGACGATGCTCGGCGCGTCGCTGTGGCGCTATGCGACGCCGGAAATCGCCGCGTTGGAAGCGTCGCTCG
ATACGCTCGGCTGGCGCGATAGCGCCGGGCCGCCGAGCGTCGAGTTCGACAGCGGCGCGAACGCATCGCGCATCGTGCCG
ATCCATCGCAGCACCTGCCGCTGGACGCGGATGATCCTGTCGGACGGCTCGGCCGCGCGGCTCGTCGAAACGCTGTAG