Detailed information of TA system TA058501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 499628..500247
• Replicon: chromosome
• Accession: NZ_CP013363
• Organism: Burkholderia diffusa strain RF2-non-BP9

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1B4C2N2
• Locus tag: WI26_RS17720
• Protein ID: WP_069227769.1
• Coordinates: 499930..500247 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: WI26_RS17715
• Protein ID: WP_006750245.1
• Coordinates: 499628..499927 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WI26_RS17690	495520..496470	+	951	WP_069226684.1	2-dehydropantoate 2-reductase	-
WI26_RS17695	496467..496859	+	393	WP_069226685.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
WI26_RS17700	496862..497320	+	459	WP_059511672.1	MaoC family dehydratase	-
WI26_RS17705	497320..498531	+	1212	WP_069226686.1	CoA transferase	-
WI26_RS17710	498539..499372	+	834	WP_059542154.1	shikimate dehydrogenase	-
WI26_RS17715	499628..499927	-	300	WP_006750245.1	putative addiction module antidote protein	Antitoxin
WI26_RS17720	499930..500247	-	318	WP_069227769.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WI26_RS17725	500633..502273	+	1641	WP_069226687.1	acetolactate synthase large subunit	-
WI26_RS17730	502302..503735	+	1434	WP_059466577.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-96)

Antitoxin

• Uncharacterized protein HI1420 (5-93)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11984.70 Da
• Isoelectric Point: 10.1539

>T58501 WP_069227769.1 NZ_CP013363:c500247-499930 [Burkholderia diffusa]
MPHSAPTFSIRTTDVFDAWFARLQDRVARRRIQARIDRLSMGNPGDWKSAGSPVVEMRVDHGPGYRVYYVRRGTIWVILL
CGGDKSTQQADIRAAHAMLERLDLE

Nucleotide

• Length: 318 bp

>T58501 NZ_CP013363:c500247-499930 [Burkholderia diffusa]
ATGCCCCACAGTGCGCCTACATTCAGTATCCGGACCACCGACGTCTTCGATGCATGGTTTGCCCGCCTGCAGGATCGCGT
CGCGCGGCGGCGCATCCAGGCACGCATCGACCGTCTGTCGATGGGCAACCCGGGCGACTGGAAATCCGCCGGCTCGCCCG
TCGTCGAAATGCGTGTCGACCACGGCCCCGGCTATCGCGTCTACTACGTCAGACGCGGAACGATATGGGTGATCCTGCTC
TGCGGCGGCGACAAGTCGACGCAACAGGCGGACATCCGCGCCGCGCACGCGATGCTCGAGCGGCTCGACCTGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10674.11 Da
• Isoelectric Point: 6.6384

>AT58501 WP_006750245.1 NZ_CP013363:c499927-499628 [Burkholderia diffusa]
MDKIKTRPWDSAEHLKTDDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT58501 NZ_CP013363:c499927-499628 [Burkholderia diffusa]
ATGGACAAGATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGACTATTTCGAGGC
CTGTCTGCAGGAGGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCCCGCGCGCGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGACTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCGTGA

Structures

Toxin

• AlphaFold DB: A0A1B4C2N2