Detailed information of TA system TA058499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1710213..1710898
• Replicon: chromosome
• Accession: NZ_CP013362
• Organism: Burkholderia diffusa strain RF2-non-BP9

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: WI26_RS07860
• Protein ID: WP_081334245.1
• Coordinates: 1710476..1710898 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A1B4BSA4
• Locus tag: WI26_RS07855
• Protein ID: WP_059467521.1
• Coordinates: 1710213..1710479 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WI26_RS07840	1705747..1707999	+	2253	WP_069225666.1	TonB-dependent siderophore receptor	-
WI26_RS07845	1708085..1708924	+	840	WP_069225667.1	N(5)-hydroxyornithine transformylase PvdF	-
WI26_RS07850	1708924..1709943	+	1020	WP_069225668.1	acetyltransferase	-
WI26_RS07855	1710213..1710479	+	267	WP_059467521.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
WI26_RS07860	1710476..1710898	+	423	WP_081334245.1	PIN domain-containing protein	Toxin
WI26_RS07865	1710941..1711846	-	906	WP_069225669.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
WI26_RS07870	1711815..1712882	-	1068	WP_069226374.1	hypothetical protein	-
WI26_RS07875	1713242..1714405	+	1164	WP_060325664.1	amidohydrolase	-
WI26_RS07880	1714573..1715112	+	540	WP_069226375.1	outer membrane protein assembly factor BamE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14656.72 Da
• Isoelectric Point: 7.8895

>T58499 WP_081334245.1 NZ_CP013362:1710476-1710898 [Burkholderia diffusa]
VTTLYMLDTNICSFIMRERPSAVLSRLQSCVNAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLDGVLSWDTA
AVDATAAIRSDLAARGTPIGANDASIAGHAIAAGAVLVTNNGREFGRVAGLSLEDWAASA

Nucleotide

• Length: 423 bp

>T58499 NZ_CP013362:1710476-1710898 [Burkholderia diffusa]
GTGACCACGCTCTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGGCCGTCCGCGGTGTTGTCCCGGCT
GCAGTCGTGCGTCAATGCGCAGCACCGGATCGTCGTGTCGGCGATTACCTATGCCGAAATGCGGTTCGGCGCGGTCGGCA
GAAAGGCATCGCCGAAGCATGCGGAACTCGTGACGGCGTTTGTCGCAAGGCTGGACGGCGTGTTGTCGTGGGATACCGCG
GCGGTCGATGCAACGGCCGCGATTCGCTCCGACCTTGCCGCACGCGGGACGCCGATCGGCGCGAACGATGCATCGATCGC
CGGCCATGCGATCGCGGCCGGTGCCGTGCTCGTCACGAACAATGGTCGCGAATTCGGCCGCGTCGCGGGGTTGTCGCTCG
AAGACTGGGCGGCGTCGGCATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9829.10 Da
• Isoelectric Point: 4.5112

>AT58499 WP_059467521.1 NZ_CP013362:1710213-1710479 [Burkholderia diffusa]
MRTVSIFKNARNQAIRIPKDMEFEGVTELEIRREGDTLLLRPVRPTWLSLASEPLADADFLVERPTVVEAGRFDLSGTDG
DAPTESGR

Nucleotide

• Length: 267 bp

>AT58499 NZ_CP013362:1710213-1710479 [Burkholderia diffusa]
ATGCGCACCGTTTCCATTTTCAAGAACGCCCGTAACCAGGCAATCCGCATCCCGAAGGACATGGAGTTCGAGGGGGTGAC
GGAACTCGAAATCCGCCGCGAGGGCGACACGCTGCTGCTGCGTCCGGTGCGGCCGACATGGCTGTCGTTGGCAAGCGAAC
CGCTGGCCGATGCCGACTTCCTCGTCGAACGACCGACTGTCGTCGAAGCGGGGCGTTTCGACCTGTCCGGCACGGATGGC
GACGCACCGACGGAGTCGGGCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B4BSA4