Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2055691..2056280 Replicon chromosome
Accession NZ_CP013344
Organism Sphingopyxis macrogoltabida strain 203N

Toxin (Protein)


Gene name vapC Uniprot ID A0A0P0DHD6
Locus tag ATM17_RS09855 Protein ID WP_054727421.1
Coordinates 2055912..2056280 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0P0DB65
Locus tag ATM17_RS09850 Protein ID WP_054727423.1
Coordinates 2055691..2055915 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ATM17_RS30335 2051530..2051907 + 378 WP_201258441.1 alpha/beta hydrolase -
ATM17_RS30340 2051927..2052253 + 327 WP_201258442.1 alpha/beta hydrolase -
ATM17_RS09825 2052250..2052912 - 663 WP_054727433.1 HAD-IA family hydrolase -
ATM17_RS09830 2052960..2054327 + 1368 WP_054727431.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
ATM17_RS09835 2054332..2054880 + 549 WP_054727430.1 GNAT family N-acetyltransferase -
ATM17_RS09840 2054877..2055194 + 318 WP_054727428.1 hypothetical protein -
ATM17_RS09845 2055196..2055660 + 465 WP_054727426.1 GNAT family N-acetyltransferase -
ATM17_RS09850 2055691..2055915 + 225 WP_054727423.1 ribbon-helix-helix domain-containing protein Antitoxin
ATM17_RS09855 2055912..2056280 + 369 WP_054727421.1 PIN domain-containing protein Toxin
ATM17_RS09860 2056298..2058121 + 1824 WP_054727419.1 glutamine--fructose-6-phosphate transaminase (isomerizing) -
ATM17_RS29455 2058251..2058964 + 714 WP_145923402.1 hypothetical protein -
ATM17_RS09865 2059202..2059873 + 672 WP_054727417.1 HAD family phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-105)

Antitoxin

Ribbon-helix-helix protein, CopG

(7-38)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13094.19 Da        Isoelectric Point: 9.1636

>T58482 WP_054727421.1 NZ_CP013344:2055912-2056280 [Sphingopyxis macrogoltabida]
MIEAQFDSDILIDALNGVEAARSEIRRAGRKSVSRISWTEVMSAADPASVKAVEAFLGCFAIEEVGDAVARRAAALRAER
KGLTMADAFVLATAQISGRILVTRNIKVFPASMPGIRVPYTL

Download         Length: 369 bp

>T58482 NZ_CP013344:2055912-2056280 [Sphingopyxis macrogoltabida]
ATGATCGAGGCGCAGTTCGACAGCGATATCCTCATCGACGCGCTGAACGGCGTCGAGGCGGCGCGCAGCGAGATTCGCCG
CGCCGGACGCAAGAGCGTCAGCCGGATCAGCTGGACCGAGGTGATGTCGGCCGCCGACCCCGCGTCGGTGAAGGCGGTCG
AGGCCTTTCTCGGCTGTTTCGCGATCGAGGAGGTCGGCGATGCGGTGGCCCGACGCGCCGCTGCGTTGAGGGCCGAACGC
AAGGGCTTGACGATGGCCGACGCCTTCGTCTTAGCAACCGCGCAAATATCGGGCCGCATCCTCGTTACGCGCAATATCAA
GGTGTTTCCGGCATCGATGCCGGGTATCCGCGTTCCCTACACGCTCTAG

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8384.38 Da        Isoelectric Point: 6.2939

>AT58482 WP_054727423.1 NZ_CP013344:2055691-2055915 [Sphingopyxis macrogoltabida]
MRFLADIPDDDVQWLDALAASQGVSRAELMRRAVSAYRADISGDAIDNGFGIWRSRDDIGDGLKYQRRLRGKRE

Download         Length: 225 bp

>AT58482 NZ_CP013344:2055691-2055915 [Sphingopyxis macrogoltabida]
ATGCGTTTCCTTGCCGATATTCCCGATGACGATGTCCAGTGGCTAGATGCGCTCGCGGCGTCGCAGGGCGTGTCGCGCGC
CGAGCTGATGCGCCGCGCGGTGAGCGCGTACCGCGCCGACATATCGGGCGACGCGATCGACAATGGGTTCGGCATATGGC
GGTCGCGTGACGATATCGGCGACGGCCTGAAATATCAGCGCCGCCTCAGGGGCAAGCGCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P0DHD6

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0DB65

References