Detailed information of TA system TA058481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 107644..109196
• Replicon: chromosome
• Accession: NZ_CP013342
• Organism: Sphingopyxis terrae subsp. terrae NBRC 15098 strain 203-1

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: A0A142VTP7
• Locus tag: AOA14_RS00505
• Protein ID: WP_062900402.1
• Coordinates: 107644..108978 (-)
• Length: 445 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: AOA14_RS00510
• Protein ID: WP_062902936.1
• Coordinates: 108981..109196 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOA14_RS00475	102846..103676	-	831	WP_062900396.1	23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ	-
AOA14_RS00480	104075..105004	-	930	WP_062900397.1	HEPN domain-containing protein	-
AOA14_RS00485	105068..105241	-	174	WP_202988342.1	hypothetical protein	-
AOA14_RS00490	105417..106157	-	741	WP_062900399.1	RES family NAD+ phosphorylase	-
AOA14_RS00495	106129..106503	-	375	WP_062900400.1	MbcA/ParS/Xre antitoxin family protein	-
AOA14_RS00500	106751..107647	-	897	WP_062900401.1	HNH endonuclease	-
AOA14_RS00505	107644..108978	-	1335	WP_062900402.1	type II toxin-antitoxin system HipA family toxin	Toxin
AOA14_RS00510	108981..109196	-	216	WP_062902936.1	helix-turn-helix domain-containing protein	Antitoxin
AOA14_RS19005	109382..109800	+	419	Protein_99	DUF2384 domain-containing protein	-
AOA14_RS00515	110301..110546	+	246	WP_062900403.1	helix-turn-helix domain-containing protein	-
AOA14_RS00520	110731..111873	+	1143	WP_202988343.1	hypothetical protein	-
AOA14_RS00525	112071..113480	+	1410	WP_082819769.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (141-372)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-44)

Sequences

Toxin

Protein

• Length: 445 a.a.
• Molecular weight: 47548.91 Da
• Isoelectric Point: 5.0917

>T58481 WP_062900402.1 NZ_CP013342:c108978-107644 [Sphingopyxis terrae subsp. terrae NBRC 15098]
VDLPVHYEDRLVATISADAQTTRLAYADAWGSSADSFPVSLAMPIRPEPYEGGQVLPWLMNLLPEGEPLRAMTRALGAAP
EDALGLIAQTGNDLAGALSIAPQRPRGEPGYRPIPDTGALERIIEELPARPFLVGEDGVSMSLAGAQEKLPVAVIDGQIA
VPINGAPSTHILKPDNPRLPGSVHNEALCMLLARRIGLNVAPVTTGVAGERSYLLVERYDRTGTGNNVRRLHQEDFCQAL
GSPPAAKYEFNGTGMRGPSIADMFAVVREHMTARDITRLLDAVIFNIAIGNVDSHAKNYSILLGPGAPQLAPLYDLMSGL
AWTNITQNHAQAIGEQRRGRHIYGRHWRRMADAAGLAARGTVQRVEQVTARLLRELPAAVEEVASMPAGSSMLEVFAKEI
EERATEVRGHAEKEGLPDTAEVPTDDVPTIGASAYSADREAGAS

Nucleotide

• Length: 1335 bp

>T58481 NZ_CP013342:c108978-107644 [Sphingopyxis terrae subsp. terrae NBRC 15098]
GTGGACCTTCCGGTTCACTATGAAGACCGCCTGGTCGCCACGATCAGCGCCGATGCGCAGACAACCCGCCTTGCCTATGC
GGATGCCTGGGGTTCATCCGCTGACAGCTTCCCTGTCTCGCTGGCCATGCCGATCCGGCCAGAGCCCTATGAGGGGGGCC
AAGTTCTTCCCTGGCTGATGAACCTCCTGCCGGAGGGCGAACCGTTGCGCGCCATGACGCGCGCGCTAGGCGCTGCACCG
GAGGACGCGCTCGGCCTGATCGCACAGACCGGCAACGATCTGGCAGGCGCGCTCAGCATCGCTCCGCAGCGGCCGCGCGG
CGAGCCCGGCTATCGCCCGATCCCGGATACCGGTGCACTGGAACGGATCATCGAGGAACTGCCAGCGCGGCCATTCCTGG
TCGGCGAGGACGGCGTCTCGATGAGCCTCGCCGGCGCGCAGGAAAAGCTGCCGGTGGCTGTCATCGACGGCCAGATTGCG
GTGCCGATCAACGGCGCGCCATCCACCCATATCCTAAAGCCCGACAATCCACGCCTACCCGGCAGCGTCCACAATGAAGC
GCTATGCATGCTTCTCGCCCGCCGCATCGGCCTCAATGTCGCGCCGGTGACAACGGGCGTTGCAGGGGAACGCAGCTACC
TGCTGGTCGAGCGCTATGACCGGACGGGTACTGGCAACAATGTCCGCCGCCTGCATCAGGAGGATTTCTGTCAGGCACTC
GGCAGCCCGCCTGCCGCCAAGTATGAATTCAACGGCACCGGAATGCGCGGCCCCTCGATCGCCGACATGTTCGCGGTTGT
CCGCGAGCATATGACGGCGCGCGACATCACCCGGCTGCTGGACGCCGTGATCTTCAATATCGCCATCGGCAACGTGGACT
CACACGCAAAGAACTACTCGATCCTGCTCGGCCCAGGTGCCCCGCAGCTTGCTCCGCTCTACGACCTGATGTCGGGCCTC
GCCTGGACCAACATCACCCAGAACCACGCCCAAGCGATCGGCGAGCAGCGCCGCGGCCGCCACATCTACGGTCGCCACTG
GCGGCGCATGGCCGACGCCGCAGGCCTGGCAGCGCGGGGAACGGTGCAGAGGGTGGAGCAGGTGACGGCACGGTTGCTGC
GCGAATTGCCCGCAGCGGTCGAGGAGGTGGCATCTATGCCAGCAGGTTCGTCGATGCTGGAGGTCTTCGCAAAAGAGATC
GAGGAGCGGGCAACAGAAGTCAGGGGACACGCCGAAAAGGAAGGCCTGCCCGACACCGCTGAAGTGCCTACGGACGACGT
GCCGACGATAGGCGCAAGCGCCTATTCTGCTGACAGGGAAGCGGGAGCGTCATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7209.34 Da
• Isoelectric Point: 10.7685

>AT58481 WP_062902936.1 NZ_CP013342:c109196-108981 [Sphingopyxis terrae subsp. terrae NBRC 15098]
MIDNATQFGAAVASARKALGLTQRELALTINSGERFIVDLEAGKPTAQLGKALAAAKAVGLRLTATGISQA

Nucleotide

• Length: 216 bp

>AT58481 NZ_CP013342:c109196-108981 [Sphingopyxis terrae subsp. terrae NBRC 15098]
ATGATTGACAACGCAACCCAATTCGGCGCAGCCGTCGCCTCGGCCCGCAAGGCCCTTGGCCTTACCCAGCGGGAGCTGGC
GCTCACCATCAACAGCGGCGAGCGCTTCATCGTCGATCTGGAAGCAGGCAAGCCCACCGCCCAACTCGGGAAGGCACTCG
CCGCCGCAAAGGCTGTCGGCTTGCGGCTCACCGCAACCGGCATTTCGCAGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A142VTP7