Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 43140..43665 Replicon plasmid pKPYL2
Accession NZ_CP013340
Organism Raoultella ornithinolytica strain Yangling I2

Toxin (Protein)


Gene name ccdB Uniprot ID A0A1D8K3Q5
Locus tag ATN83_RS26490 Protein ID WP_006788214.1
Coordinates 43360..43665 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag ATN83_RS26485 Protein ID WP_006788213.1
Coordinates 43140..43358 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ATN83_RS26450 38777..39301 - 525 Protein_37 IS5-like element ISKpn26 family transposase -
ATN83_RS26455 39337..40776 - 1440 Protein_38 IS66 family transposase -
ATN83_RS26460 40841..41545 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
ATN83_RS26465 41631..41771 + 141 WP_045286941.1 helix-turn-helix domain-containing protein -
ATN83_RS26470 41869..42285 - 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin -
ATN83_RS26475 42282..42512 - 231 WP_021312767.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ATN83_RS28790 42566..42970 + 405 Protein_43 hypothetical protein -
ATN83_RS26485 43140..43358 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ATN83_RS26490 43360..43665 + 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB Toxin
ATN83_RS28655 44351..44836 + 486 Protein_46 hypothetical protein -
ATN83_RS26500 45072..45866 + 795 WP_016240356.1 site-specific integrase -
ATN83_RS28360 46306..46485 - 180 WP_006788217.1 hypothetical protein -
ATN83_RS26505 46605..47231 - 627 WP_016946792.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..140936 140936


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11687.46 Da        Isoelectric Point: 7.2015

>T58477 WP_006788214.1 NZ_CP013340:43360-43665 [Raoultella ornithinolytica]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRLMTTDMASVPASVTGEEV
ADLRHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T58477 NZ_CP013340:43360-43665 [Raoultella ornithinolytica]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGGCGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAGCTCTATCCGGTGG
TGCACATCGGGGACGAGAGCTGGCGCCTGATGACCACGGACATGGCCAGCGTCCCGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCCGCCACCGGGAAAATGACATCAAAAACGCAATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT58477 WP_006788213.1 NZ_CP013340:43140-43358 [Raoultella ornithinolytica]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT58477 NZ_CP013340:43140-43358 [Raoultella ornithinolytica]
ATGAAGCAGCGCATCACCGTCACCATCGACAGCGACAGCTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAAGCCCGACGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCTG
AGGTTGCCCGGTTTATTGAAATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8K3Q5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References