Detailed information of TA system TA058474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4751699..4752275
• Replicon: chromosome
• Accession: NZ_CP013338
• Organism: Raoultella ornithinolytica strain Yangling I2

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: ATN83_RS22100
• Protein ID: WP_064357557.1
• Coordinates: 4751988..4752275 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: ATN83_RS22095
• Protein ID: WP_064357555.1
• Coordinates: 4751699..4752001 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATN83_RS22080	4747637..4747972	+	336	WP_044348942.1	endoribonuclease SymE	-
ATN83_RS22085	4748300..4750276	+	1977	WP_162893513.1	AAA family ATPase	-
ATN83_RS22090	4750276..4751598	+	1323	WP_044348938.1	mcrBC 5-methylcytosine restriction system component family protein	-
ATN83_RS22095	4751699..4752001	-	303	WP_064357555.1	BrnA antitoxin family protein	Antitoxin
ATN83_RS22100	4751988..4752275	-	288	WP_064357557.1	BrnT family toxin	Toxin
ATN83_RS22105	4752392..4752811	-	420	WP_015585224.1	FosA family fosfomycin resistance glutathione transferase	-
ATN83_RS22110	4752805..4753713	-	909	WP_064357559.1	LysR family transcriptional regulator	-
ATN83_RS22115	4753801..4754583	+	783	WP_064357561.1	NAD(P)H-dependent oxidoreductase	-
ATN83_RS22120	4754731..4755309	+	579	WP_004857495.1	TetR/AcrR family transcriptional regulator	-
ATN83_RS22125	4755453..4756265	+	813	WP_004857492.1	winged helix-turn-helix domain-containing protein	-
ATN83_RS22130	4756262..4756780	+	519	WP_015585229.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (35-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.54 Da
• Isoelectric Point: 7.5725

>T58474 WP_064357557.1 NZ_CP013338:c4752275-4751988 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRHDRYENGEHRWQTIGLVHGVIVVLVAHSIRFESGTEVIRIIS
ARKADKKERSRYEHG

Nucleotide

• Length: 288 bp

>T58474 NZ_CP013338:c4752275-4751988 [Raoultella ornithinolytica]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAGCAAGCAATCTAAGAAAACATGGGATTCGCTTCGAGGAGGCGGT
ACTGGTTTTTGACGATCCGCAGCATCTTTCCCGACATGATCGCTATGAAAACGGTGAGCATCGATGGCAAACCATCGGGC
TTGTTCATGGCGTGATTGTTGTTCTTGTGGCGCATTCGATCCGATTTGAAAGTGGAACGGAGGTCATTCGTATTATCAGT
GCCCGGAAGGCGGACAAAAAAGAGAGGAGTCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11509.97 Da
• Isoelectric Point: 7.0165

>AT58474 WP_064357555.1 NZ_CP013338:c4752001-4751699 [Raoultella ornithinolytica]
MSMVKHKRDNPTALTAQREDELKALAKKSDDDIDYSDIPSSDDEPWSEAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMNRDLGEK

Nucleotide

• Length: 303 bp

>AT58474 NZ_CP013338:c4752001-4751699 [Raoultella ornithinolytica]
ATGAGCATGGTTAAACATAAACGCGATAACCCGACAGCGCTTACCGCTCAGCGTGAAGACGAACTCAAAGCGCTGGCGAA
AAAATCAGATGATGACATTGATTACAGCGATATCCCCTCTTCGGACGATGAGCCGTGGTCAGAGGCGGTTCGCGGTAAAT
TTTATCGGCCATTAAAAACGCAGGCCTCTGTGCGCATCGATGCAGATGTGATGGAGTGGCTTAAACGCCCGGGAAAAGGC
TATCAAACGCGGCTGAATGCGATTTTAAGAGAAGCGATGAACCGCGATCTGGGTGAGAAATAA